A top-down proteomic approach reveals a salivary protein profile classifying Parkinson’s disease with respect to Alzheimer’s disease patients and to healthy controls

ABSTRACT: Parkinson’s disease (PD) is a complex neurodegenerative disease (ND) with heterogeneous clinical conditions, including progressive motor and non-motor symptoms and cognitive impairment, because of the loss of dopaminergic and serotoninergic neurons across the brain. Brain tissue and CSF are elective samples in proteomic studies of NDs because appropriate to individuate central biomarkers, however, brain tissues are available post-mortem and CSF collection is very invasive and not well-accepted by the patients. Thus, peripheral biomarkers represent a great advantage. In the present study we investigated a panel of salivary peptides/proteins that are detectable by a top-down proteomic approach based on high performance liquid chromatography separation coupled to electrospray-ion trap mass spectrometry (HPLC-ESI-IT-MS) platform. The approach to study the acidic soluble proteome of human saliva was standardized in our previous studies for detecting and quantifying hundreds salivary peptides/proteins. In this way, it is possible to obtain a profiling of the naturally occurring salivary proteome, including isoforms and post-translational modifications (PTMs), to compare an unlimited number of samples and to quantify proteins by a label-free method.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

DISEASE(S): Parkinson's Disease

SUBMITTER: Cristina Contini  

LAB HEAD: Tiziana Cabras

PROVIDER: PXD041787 | Pride | 2026-10-05

REPOSITORIES: Pride