PXD041814 - immunoprecipitation coupled with a mass spectrometry (MS) approach to identify cellular proteins associated with MOV10

Dataset Information

Immunoprecipitation coupled with a mass spectrometry (MS) approach to identify cellular proteins associated with MOV10

ABSTRACT: To understand the detailed mechanism underlying MOV10-mediated restriction of LINE-1 retrotransposition, we first performed immunoprecipitation coupled with a mass spectrometry (MS) approach to identify cellular proteins associated with MOV10.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Qian Liu

LAB HEAD: shan cen

PROVIDER: PXD041814 | Pride | 2023-08-30

REPOSITORIES: Pride

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Publications

MOV10 recruits DCP2 to decap human LINE-1 RNA by forming large cytoplasmic granules with phase separation properties.

Liu Qian Q Yi Dongrong D Ding Jiwei J Mao Yang Y Wang Shujie S Ma Ling L Li Quanjie Q Wang Jing J Zhang Yongxin Y Zhao Jianyuan J Guo Saisai S Liu Zhenlong Z Guo Fei F Zhao Dongbing D Liang Chen C Li Xiaoyu X Peng Xiaozhong X Cen Shan S

EMBO reports 20230712 9

Long interspersed element 1 (LINE-1) is the only active autonomous mobile element in the human genome. Its transposition can exert deleterious effects on the structure and function of the host genome and cause sporadic genetic diseases. Tight control of LINE-1 mobilization by the host is crucial for genetic stability. In this study, we report that MOV10 recruits the main decapping enzyme DCP2 to LINE-1 RNA and forms a complex of MOV10, DCP2, and LINE-1 RNP, exhibiting liquid-liquid phase separat ...[more]

PMID: 37437058

Dataset Information

Immunoprecipitation coupled with a mass spectrometry (MS) approach to identify cellular proteins associated with MOV10

Publications

MOV10 recruits DCP2 to decap human LINE-1 RNA by forming large cytoplasmic granules with phase separation properties.

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