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Perforin-2 is a pore-forming effector of endocytic escape in cross-presenting dendritic cells

ABSTRACT: This study searched for cell-type specific effectors of endocytic escape in dendritic cells and identified a pore-forming protein, perforin-2 (encoded by Mpeg1), as a dedicated effector exclusive to cross-presenting cells. Whole cell proteomics identified differences in the abundance of tryptic and semi-tryptic (cleaved) perforin-2 peptides between control MutuDCs and cells treated with BafA, CpG or with knockout of asparagine endopeptidase (AEP). This revealed how perforin-2 undergoes proteolytic cleavage releasing its pore forming domain into the organellar lumen.

INSTRUMENT(S): Orbitrap Exploris 480, Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Dendritic Cell

DISEASE(S): Disease Free

SUBMITTER: Alexandra Davies

LAB HEAD: Georg H. H. Borner

PROVIDER: PXD041861 | Pride | 2023-06-27

REPOSITORIES: Pride

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