Dataset Information

Pak3 interactors in human cells during starvation

ABSTRACT: Human Pak3-T421E cDNA with a flag tag was transfected into HEK293 cells using Lipofectomine 2000, After 72 hrs of incubation, PAK3 interactors were pulldwon with FLAG antibody. A sample without PAK3 overexpression was used as a negative control. Changes in PAK3 interactions between basal and starvation conditions were studied.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Early Embryonic Cell

SUBMITTER: Andrea Ruiz-Velasco Hernandez

LAB HEAD: Wei Liu

PROVIDER: PXD041986 | Pride | 2023-05-16

REPOSITORIES: Pride

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			Action	DRS
	20210430_AR_01.raw	Raw
	20210430_AR_02.raw	Raw
	20210430_AR_03.raw	Raw
	20210430_AR_swiss_trembl_human_01.dat	Other
	20210430_AR_swiss_trembl_human_02.dat	Other

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Project description:While the majority of protein-coding genes in the human genome have been identified, the location of the regulatory sequences that control their expression in time and space remains poorly defined. The importance of identifying these elements is underscored by a growing number of studies (such as GWAS) supporting a relationship between variation in non-coding sequences and human disease. M-BM- emonstrated that ChIP-Seq with p300 directly on human or mouse tissues represents a powerful method to identify the location and tissue-specific activity pattern of enhancers in the genome.M-BM- a highly specific chromatin mark for the prediction of in vivo enhancers, it is not without limitations. M-BM- comparison of p300-bound regions with previously generated enhancer sets shows that the majority of enhancers are activated independently of p300. To attempt to identify a larger proportion of enhancers in vivo, we have focused on several additional transcriptional coactivators that are hypothesized to be associated with active tissue-specific enhancers. M-BM- limited availability of ChIP-seq grade antibodies for these coactivators, FLAG-tag knock-in mice were generated and ChIP-seq using a FLAG antibody was performed on various tissues from e11.5 mouse embryos. Indeed, as these mouse lines have become available we have validated their valuable role as marks for transcriptional enhancers in vivo.M-BM- studies are expected to further expand the catalogue of in vivo functional enhancers, which will aid in the decoding of the regulatory genome and provide new insights into the role of gene regulation in human biology and disease. In addition to FLAG data, this accession includes histone acetylation / methylation ChIP-seq data from e11.5 mouse embryonic tissues. Histone ChIP-seq data was used in combination with FLAG data for various computational analyses. Examination of FLAG-labeled transcriptional coactivator binding in mouse embryonic stage 11.5 and embryonic stem cells

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Pak3 interactors in human cells during starvation

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