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Label-free quantitative plasma membrane proteome analysis of Candida glabrata wild-type and Cgvps34∆ (lacks phosphatidylinositol 3- phosphate kinase, CgVps34) strains. Label-free quantitative plasma membrane proteome analysis of Candida glabrata wild-type and Cgvps34∆ (lacks phosphatidylinositol 3- phosphate kinase, CgVps34) strains.

ABSTRACT: The project is aimed at characterizing the effect of loss of phosphatidylinositol 3- phosphate kinase (CgVps34) on the plasma membrane proteome of Candida glabrata.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Candida Glabrata (yeast) (torulopsis Glabrata)

SUBMITTER: Rupinder Kaur

LAB HEAD: Rupinder Kaur

PROVIDER: PXD042056 | Pride | 2024-02-19

REPOSITORIES: Pride

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Phosphatidylinositol 3-phosphate regulates iron transport via PI3P-binding CgPil1 protein.

Askari Fizza F Vasavi Bhogadi B Kaur Rupinder R

Cell reports 20230724 8

Iron homeostasis, which is pivotal to virulence, is regulated by the phosphatidylinositol 3-kinase CgVps34 in the human fungal pathogen Candida glabrata. Here, we identify CgPil1 as a phosphatidylinositol 3-phosphate (PI3P)-binding protein and unveil its role in retaining the high-affinity iron transporter CgFtr1 at the plasma membrane (PM), with PI3P negatively regulating CgFtr1-CgPil1 interaction. PI3P production and its PM localization are elevated in the high-iron environment. Surplus iron a ...[more]

PMID: 37490387

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