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Phosphatidylinositol 3-phosphate (PI3P) interactome analysis under varied iron conditions in Candida glabrata.

ABSTRACT: The project is aimed at identifying proteins that interact with PI3P in low-iron, regular-iron and high-iron grown Candida glabrata cells.

INSTRUMENT(S): LTQ Orbitrap Velos Pro

ORGANISM(S): Candida Glabrata (yeast) (torulopsis Glabrata)

SUBMITTER: Rupinder Kaur

LAB HEAD: Rupinder Kaur

PROVIDER: PXD042058 | Pride | 2024-02-19

REPOSITORIES: Pride

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Phosphatidylinositol 3-phosphate regulates iron transport via PI3P-binding CgPil1 protein.

Askari Fizza F Vasavi Bhogadi B Kaur Rupinder R

Cell reports 20230724 8

Iron homeostasis, which is pivotal to virulence, is regulated by the phosphatidylinositol 3-kinase CgVps34 in the human fungal pathogen Candida glabrata. Here, we identify CgPil1 as a phosphatidylinositol 3-phosphate (PI3P)-binding protein and unveil its role in retaining the high-affinity iron transporter CgFtr1 at the plasma membrane (PM), with PI3P negatively regulating CgFtr1-CgPil1 interaction. PI3P production and its PM localization are elevated in the high-iron environment. Surplus iron a ...[more]

PMID: 37490387

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Label-free quantitative plasma membrane proteome analysis of Candida glabrata wild-type and Cgvps34∆ (lacks phosphatidylinositol 3- phosphate kinase, CgVps34) strains. Label-free quantitative plasma membrane proteome analysis of Candida glabrata wild-type and Cgvps34∆ (lacks phosphatidylinositol 3- phosphate kinase, CgVps34) strains.

Project description:The project is aimed at characterizing the effect of loss of phosphatidylinositol 3- phosphate kinase (CgVps34) on the plasma membrane proteome of Candida glabrata.

2024-02-19 | PXD042056 | Pride

Total membrane proteome analysis of Candida glabrata wild-type and eleven CgYapsins-deficient strain Cgyps1-11∆.

Project description:The project is aimed at characterizing the effect of loss of a family of eleven aspartyl proteases (Cg Yapsins) on the global membrane proteome of Candida glabrata.

2022-04-04 | PXD029614 | Pride

Label-free quantitative total membrane proteome analysis of Candida glabrata wild-type and eleven CgYapsins-deficient strain Cgyps1-11∆.

Project description:The project is aimed at characterizing the effect of loss of a family of eleven aspartyl proteases (Cg Yapsins) on the abundance of proteins in the total membrane factions of Candida glabrata.

2022-04-04 | PXD029629 | Pride

A unique iron homeostasis regulatory circuit with reciprocal roles in Candida albicans commensalism and pathogenesis [ChIP_chip]

Project description:The mammalian gastrointestinal tract and the bloodstream are highly disparate biological niches, and yet certain commensal-pathogenic microorganisms are able to thrive in both environments. Here, we report the evolution of a unique transcription circuit in the yeast, Candida albicans, which determines its fitness in both host niches. Our comprehensive analysis of the DNA-binding proteins that regulate iron uptake by this organism suggests the evolutionary intercalation of a transcriptional activator called Sef1 between two broadly conserved transcriptional repressors, Sfu1 and Hap43. The Sef1 activator of iron uptake genes promotes virulence in a mouse model of bloodstream infection, whereas the Sfu1 repressor is dispensable for virulence but promotes gastrointestinal commensalism. We propose that the ability to alternate between genetic programs conferring resistance to iron depletion in the bloodstream versus iron toxicity in the gut may be a fundamental attribute of gastrointestinal commensal-pathogens. ChIP analyses to profile genome-wide of distribution of Sef1, Sfu1 and Hap43 in response to various iron availability. 12 independent ChIP experiments were performed on 6 biological replicates of the untagged control and 2 biological replicates each of Sef1-Myc, Sfu1-Myc, and Hap43-Myc.

2011-07-12 | E-GEOD-30591 | biostudies-arrayexpress

Candida albicans Microarray studies: CAP2 (Wild type) vs. cap2D (Homozygous null mutant)

Project description:Transcriptome profiling to identify Cap2/Hap43 regulons in the human fungal pathogen Candida albicans: Wild type vs. cap2D grown in iron-depleted medium Two-condition experiment, WT vs. cap2D cells. Biological replicates: Wild-type and cap2D, independently grown in iron-depleted medium and harvested. One replicate per array. Hybridization was repeated (technical replicate) for each biological replicate.

2011-05-24 | E-GEOD-28429 | biostudies-arrayexpress

Phosphoproteome Analysis of Magnaporthe oryzae During Appressorium Development

Project description:The rice pathogen, Magnaporthe oryzae, undergoes a complex developmental process leading to formation of an appressorium prior to plant infection. In an effort to better understand phosphoregulation during appressorium development, a mass spectrometry-based study was undertaken. Phosphosites were identified from phosphoproteins from mycelia, conidia, germlings, and appressoria of the wild type and a ckpA mutant. The cyclic-AMP dependent protein kinase A (CPKA) is required for initial perception of the leaf surface, development of functional appressoria, and timely mobilization of storage reserves in conidia, including glycogen and lipid bodies.

2015-05-13 | PXD001389 | Pride

Interactome identification of a glycosylphosphatidylinositol-linked aspartyl protease, CgYps1, in the pathogenic yeast Candida glabrata

Project description:The major goal of this project is to identify proteins that interact with CgYps1 aspartyl protease in logarithmic-phase Candida glabrata cells.

2021-02-08 | PXD022766 | Pride

Interactome identification of Histones H3 and H4 in the presence and absence of methyl methanesulfonate (MMS) in the pathogenic yeast Candida glabrata

Project description:The main goal of this project is to identify proteins in Candida glabrata that interact with histones H3 (CgHht) and H4 (CgHhf) under normal and MMS-treatment conditions.

2020-02-21 | PXD016867 | Pride

Iron deficiency and recovery in yeast: a quantitative proteomics approach

Project description:A model used to study iron deficiency is yeast, Saccharomyces cerevisiae. Here, we used quantitative (phospho)proteomics to explore the early (4h and 6h) and late (12h) response to ID. We showed that metabolic pathways like the Krebs cycle, amino acid and ergosterol biosynthesis were affected by ID. In addition, during the late response, several proteins related to the ubiquitin-proteasome system and autophagy were upregulated. We also explored the proteomic changes during a recovery period after 12h of ID. We found that several proteins recovered their steady-state levels, but some others such as cytochromes did not recover during the time tested. Our results highlight the complex proteome changes occurring during ID and recovery. This study constitutes a valuable dataset for researchers interested in iron biology, offering a temporal proteomic dataset for ID, as well as a compendium the proteomic changes associated with episodes of iron recovery

2022-05-19 | PXD023484 | Pride

Candida glabrata transcription profile when grown under Niacin starvation or in human urine

Project description:The transcription profile of Candida glabrata grown under two different Niacin limitation conditions were determined. Condition 1 is comparing log phase C. glabrata cells (O.D. 0.5-0.6) grown in synthetic medium containing 0.016 uM versus 3.25 uM nicotinic acid (NA), a common form of Niacin. The NA concentration of 3.25 uM is the standard concentration in synthetic complete (SC) medium. Condition 2 is comparing log phase C. glabrata cells (O.D. 0.4-0.6) grown in 3 individual human urine samples (supplemented with 2% glucose) versus in SC medium. Keywords: transcriptional profiling by microarray GSM151863, GSM151869 are dye-swap experiment #1 and GSM151880, GSM151881 are dye-swap experiment #2 for Niacin limitation condition 1. GSM151934, GSM152116 are dye-swap experiment #1, GSM152118, GSM152119 are dye-swap experiment #2 and GSM152130, GSM152131 are dye-swap experiment #3 for Niacin limitation condition 2.

2010-06-05 | E-GEOD-6582 | biostudies-arrayexpress

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