Dataset Information

Comparative proteome profiling of Egg, Larva and Adult of Hermetia illucens (Diptera: Stratiomyidae)

ABSTRACT: The black soldier fly (BSF), Hermetia illucens, is the insect with the greater potential to contribute to sustainable human development due to its nutritional characteristics, low environmental impact, or bioremediation ability. This study characterized and quantified the proteome of three stages of BSF: Egg, Larva, and Adult, using a Bottom-Up Proteomics strategy. A total of 6,116 proteins were identified across all BSF development stages. The processes related to Information processing, like Chromatin structure, Replication, Transcription, Translation, and Cell cycle, were notably more abundant in Egg than Larva and Adult. Still, the metabolic processes, like Amino acid, Carbohy-drate, Lipid, and Nucleotide metabolism, were more abundant in Larva and Adult than Egg. The quantitative analysis revealed four expression clusters of proteins that participate in amino acid transport and metabolism, carbohydrate metabolism, coenzyme transport and metabolism, energy production, inorganic ion transport and metabolism, lipid metabolism, posttranscriptional modi-fication and translation. Furthermore, the detection of xenobiotic detoxification proteins belonging to P450s, esterases, and GSTs in BSF suggests detoxification of exogenous molecules. The overview of the BSF proteome provided information for further investigations as biotechnological applica-tions based on newly identified detoxification enzymes or practical applications such as enhancing rearing practices with new diet formulations or bioremediation wastes.

INSTRUMENT(S):

ORGANISM(S): Hermetia Illucens

TISSUE(S): Whole Body

SUBMITTER: Guillermo Nourdin

LAB HEAD: Elard Koch

PROVIDER: PXD042131 | Pride | 2025-12-29

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	BSF_Adult_R1_200_ng_Slot2-6_1_7222.csv	Csv
	BSF_Adult_R1_200_ng_Slot2-6_1_7222.d.zip	Other
	BSF_Adult_R2_200_ng_Slot2-7_1_7223.csv	Csv
	BSF_Adult_R2_200_ng_Slot2-7_1_7223.d.zip	Other
	BSF_Adult_R3_200_ng_Slot2-8_1_7224.csv	Csv

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Publications

Comparative proteome profiling of egg, larva and adult Hermetia illucens (Diptera: Stratiomyidae).

Saldivia Pablo P Amouroux Paul P Nourdin Guillermo G Emhart Cristian C Au María Cristina MC Delgado Rocío R Hernández Mauricio M Vargas Cristian C

Scientific reports 20251124 1

The black soldier fly (BSF), Hermetia illucens, is the insect with the greatest potential to contribute to sustainable human development because of its nutritional characteristics, low environmental impact, and bioremediation ability. In this study, we characterized and quantified the proteome of three BSF stages: egg, larva, and adult, using bottom-up proteomics. A total of 6,116 proteins were identified across all BSF developmental stages. Processes related to information processing, such as c ...[more]

PMID: 41286339

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Project description:The bloodstream forms of Trypanosoma brucei (BSF), responsible for the sleeping sickness, primarily evaluate in the blood of its mammalian hosts. The skin and the adipose tissues were recently identified as additional major sites for the parasite proliferation and transmission through its insect vector blood ingestion. Glucose is the only carbon source used by the blood parasites to feed its central metabolism, however, the metabolic behavior of the tissue-adapted parasites has not yet been addressed. Since production of glycerol is an important primary function of adipocytes, we have adapted the BSF trypanosomes to glucose-depleted and glycerol-rich growth medium (CMM_Gly/GlcNAc) and compared its metabolism and proteome with standard glucose-rich growth conditions (CMM_Glu). BSF consume 2-times more oxygen per carbon consumed in CMM_Gly/GlcNAc and is 11.5-times more sensitive to SHAM, a specific inhibitor of the alternative oxidase, which is consistent with the expected absolute requirement of the mitochondrial respiratory chain activity to convert glycerol into dihydroxyacetone phosphate. Metabolomic analyses by mass spectrometry showed that 13C-labeled glycerol is incorporated into hexose phosphates through gluconeogenesis. After several weeks of growth in CMM_Gly/GlcNAc, BSF adapt their metabolism by increasing and decreasing the rate of glycerol and glucose consumption, respectively. As expected RNAi-mediated down-regulation of glycerol kinase expression abolished glycerol degradation and is lethal for BSF growth in CMM_Gly/GlcNAc. However, the glycerol kinase activity is 7.8-fold decreased in CMM_Gly/GlcNAc, as confirms by western blotting and proteomic analyses, which suggests that the huge excess of glycerol kinase, which is not necessary for glycerol metabolism, is required for another yet undetermined non-essential function in glucose rich-conditions. Altogether these data shows that BSF trypanosomes are well adapted to glycerol-rich conditions, which could be encountered by the parasite in extravascular niches, such as skin and adipose tissues.