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PolQ peptide 4S-P (1482-P/86-P/88-P/93-P) binding partners identification

ABSTRACT: We characterized residus of PolQ which are phosphorylated during mitotis. We aimed to identify the partners of the phosphorylated residus of PolQ.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Vanessa Masson

LAB HEAD: Damarys Loew

PROVIDER: PXD042167 | Pride | 2023-09-18

REPOSITORIES: Pride

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Polθ is phosphorylated by PLK1 to repair double-strand breaks in mitosis.

Gelot Camille C Kovacs Marton Tibor MT Miron Simona S Mylne Emilie E Haan Alexis A Boeffard-Dosierre Liza L Ghouil Rania R Popova Tatiana T Dingli Florent F Loew Damarys D Guirouilh-Barbat Josée J Del Nery Elaine E Zinn-Justin Sophie S Ceccaldi Raphael R

Nature 20230906 7978

DNA double-strand breaks (DSBs) are deleterious lesions that challenge genome integrity. To mitigate this threat, human cells rely on the activity of multiple DNA repair machineries that are tightly regulated throughout the cell cycle1. In interphase, DSBs are mainly repaired by non-homologous end joining and homologous recombination2. However, these pathways are completely inhibited in mitosis3-5, leaving the fate of mitotic DSBs unknown. Here we show that DNA p ...[more]

PMID: 37674080

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