Multi-omics analysis of mutagenized S. rimosus reveals synergistic changes that drive oxytetracycline production towards industrial efficiency and opens a new route to heterologous polyketides

ABSTRACT: The present work provides a multi-omics systems-wide view on S. rimosus. Using genomics, transcriptomics, proteomics, and metabolomics, we compared the wild type with an OTC-overproducing derivative, previously obtained by classical mutagenesis. The integration of the data provided a deep insight into the underlying metabolic and regulatory networks that mediate high-level OTC formation. Strikingly, the overproducer revealed a synergistically activated supply of acetyl-CoA and malonyl CoA and increased abundance of various CoA thioesters.

INSTRUMENT(S):

ORGANISM(S): Streptomyces Rimosus Subsp. Rimosus

SUBMITTER: Christian Rückert-Reed  

LAB HEAD: Jörn Kalinowski

PROVIDER: PXD042182 | Pride | 2024-01-26

REPOSITORIES: Pride