Identification of the proteins that are associated with ALKBH5 by an affinity purification and mass spectrometry (MS) analysis in human 293T cells.

ABSTRACT: Our proteomics analysis is for characterization of ALKBH5 protein interactions in 293T cells. We expressed HA-tagged ALKBH5 in 293T cells, prepared nuclear extracts, and subjected them to affinity purification using an anti-HA antibody, followed by mass spectrometry analysis of the purified protein.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Qin Xiao yang  

LAB HEAD: Qin Xiaoyang

PROVIDER: PXD042183 | Pride | 2023-07-26

REPOSITORIES: Pride