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Development and systematic evaluation of decellularization protocols for rat diaphragms

ABSTRACT: We decellularized rat diaphragms with the main detergents 1% or 0.1% sodium dodecyl sulfate (SDS) and 4% sodium deoxycholate (SDC) by orbital shaking (OS) or retrograde perfusion (RP) through the vena cava. Our systematic comparison entails evaluation of decellularized diaphragmatic samples by (1) quantitative analysis including DNA quantification and biomechanical testing, (2) qualitative and semi-quantitative analysis by proteomics, as well as (3) qualitative assessment with macroscopic and microscopic evaluation by histological staining, immunohistochemistry and scanning electron microscopy.

INSTRUMENT(S): impact II

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Diaphragm

SUBMITTER: Karl Hillebrandt

LAB HEAD: Karl Herbert Hillebrandt

PROVIDER: PXD042219 | Pride | 2024-05-07

REPOSITORIES: Pride

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Development and systematic evaluation of decellularization protocols in different application models for diaphragmatic tissue engineering.

Andreas Marco N MN Boehm Agnes K AK Tang Peter P Moosburner Simon S Klein Oliver O Daneshgar Assal A Gaßner Joseph M G V JMGV Raschzok Nathanael N Haderer Luna L Wulsten Dag D Rückert Jens-Carsten JC Spuler Simone S Pratschke Johann J Sauer Igor M IM Hillebrandt Karl H KH

Biomaterials advances 20230605

<h4>Background</h4>Tissue engineered bioscaffolds based on decellularized composites have gained increasing interest for treatment of various diaphragmatic impairments, including muscular atrophies and diaphragmatic hernias. Detergent-enzymatic treatment (DET) constitutes a standard strategy for diaphragmatic decellularization. However, there is scarce data on comparing DET protocols with different substances in distinct application models in their ability to maximize cellular removal while mini ...[more]

PMID: 37418932

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