Integrative Analysis of Transcriptome and Proteome for a new Rumen-derived Bacillus Licheniform in regulating microenvrionment Treating with Different Carbon Sources
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ABSTRACT: Bacillus licheniformis (B. licheniformis) is a microorganism with a wide range of probiotic properties and applications. Isolation and identification of novel strains is a major aspect of microbial research. Besides, the role of different carbon sources affects B. licheniformis in regulating micro-environment and the mechanisms need to be further investigated. In this study, we first isolated and identified a new strain of B. licheniformis from bovine rumen fluid. Microcrystalline cellulose (MC) and cellobiose (CB) as the certain carbon sources to treat strain. Further, a combination of transcriptome and proteome analyses was used to different carbon sources effects. The results showed that B. licheniformis ABC transporter proteins, antibiotic synthesis, flagellar assembly, cellulase-related pathways and proteins were significantly up-regulated in the MC treatment compared to the CB treatment, and lactate metabolism was inhibited. In addition, MC was used as a certain carbon source to improve bacterial inhibition of B. licheniformis, its own disease resistance and to regulate the rumen micro-environment. In conclusion, our research provides a potential new probiotic for feed research and a theoretical basis for investigating the mechanisms by which bacteria respond to different carbon sources.
INSTRUMENT(S): Q Exactive
ORGANISM(S): Bacillus Licheniformis
TISSUE(S): Cell Culture
SUBMITTER: Yuchen Cheng
LAB HEAD: Xiaofeng Xu
PROVIDER: PXD042310 | Pride | 2024-01-26
REPOSITORIES: Pride
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