Proteomics

Dataset Information

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Surface Proteomics of ADAM15 Knock-Down cells


ABSTRACT: ADAM15 is a member of the disintegrin-metalloproteinase family of sheddases, which plays a role in several biological processes including cartilage homeostasis. Contrary to well-characterized ADAMs, little is known about ADAM15 substrates or how the enzyme exerts its biological functions. Herein, we used “surface-spanning enrichment with click-sugars (SUSPECS)” proteomics to identify ADAM15 substrates and/or proteins regulated by the proteinase at the surface of chondrocyte-like cells. ADAM15-silencing significantly altered membrane levels of 13 proteins, but none of these was a canonical substrate. We used orthogonal techniques to validate ADAM15 effects on 3 of these proteins which have known roles in cartilage homeostasis. This confirmed that ADAM15-silencing increased levels of PDCD1LG2 and reduced levels of vasorin and SLC26A2 through an unknown post-translational mechanism.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Fibroblast

SUBMITTER: Stephan Mueller  

LAB HEAD: Simone Dario Scilabra

PROVIDER: PXD042402 | Pride | 2023-06-13

REPOSITORIES: Pride

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