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Autophagy protein 5 controls flow-dependent endothelial functions

ABSTRACT: In this project two sets of proteomic experiments were performed: (1) Autophagosome content profiling: Autophagosomes were isolated from cultured primary lung endothelial cells of LC3-GFP and WT mice using anti-GFP uMACS (n=3 biological replicates). (2) Expression proteomics: Protein lysates from endothelial cells of n=3 young and old WT mice, young and old vecCRE-ATG5flox mice (ATG5 KO mice).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Lung, Lung Endothelial Cell

SUBMITTER: Joern Dengjel

LAB HEAD: Joern Dengjel

PROVIDER: PXD042431 | Pride | 2023-08-07

REPOSITORIES: Pride

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Autophagy protein 5 controls flow-dependent endothelial functions.

Nivoit Pierre P Mathivet Thomas T Wu Junxi J Salemkour Yann Y Sankar Devanarayanan Siva DS Baudrie Véronique V Bourreau Jennifer J Guihot Anne-Laure AL Vessieres Emilie E Lemitre Mathilde M Bocca Cinzia C Teillon Jérémie J Le Gall Morgane M Chipont Anna A Robidel Estelle E Dhaun Neeraj N Camerer Eric E Reynier Pascal P Roux Etienne E Couffinhal Thierry T Hadoke Patrick W F PWF Silvestre Jean-Sébastien JS Guillonneau Xavier X Bonnin Philippe P Henrion Daniel D Dengjel Joern J Tharaux Pierre-Louis PL Lenoir Olivia O

Cellular and molecular life sciences : CMLS 20230718 8

Dysregulated autophagy is associated with cardiovascular and metabolic diseases, where impaired flow-mediated endothelial cell responses promote cardiovascular risk. The mechanism by which the autophagy machinery regulates endothelial functions is complex. We applied multi-omics approaches and in vitro and in vivo functional assays to decipher the diverse roles of autophagy in endothelial cells. We demonstrate that autophagy regulates VEGF-dependent VEGFR signaling and VEGFR-mediated and flow-me ...[more]

PMID: 37460898

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