Proteomics

Dataset Information

0

Phosphoproteomic dataset comparing wild type, bob1-1 and bob1-1dbf4∆ cells in M-phase arrested S.cerevisiae.


ABSTRACT: DNA double-strand breaks (DSBs) can be repaired by several pathways. In eukaryotes, repair pathway choice – the cellular decision making underlying DSB repair – occurs at the level of DSB resection and is controlled by the cell cycle. Upon cell cycle-dependent activation, cyclin-dependent kinases (CDKs) phosphorylate resection proteins and thereby stimulate DSB resection and repair by homologous recombination (HR). We uncovered a novel role for the Dbf4-dependent kinase Cdc7 (DDK) in regulating HR and DNA end resection. We therefore analyzed phosphorylation substrates of DDK by phosphoproteomics, where we compared wild type, bob1-1 and bob1-1dbf4∆ cells in M-phase arrested S.cerevisiae.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Barbara Steigenberger  

LAB HEAD: Prof. Dr. Boris Pfander

PROVIDER: PXD042607 | Pride | 2024-02-15

REPOSITORIES: Pride

Similar Datasets

2024-02-22 | GSE233549 | GEO
2019-02-26 | PXD012045 | Pride
2014-07-21 | E-GEOD-52970 | biostudies-arrayexpress
2023-03-11 | PXD031357 | Pride
2019-11-12 | PXD014441 | Pride
2022-02-04 | PXD031311 | Pride
2021-11-02 | PXD028324 | Pride
2014-03-23 | E-MTAB-1241 | biostudies-arrayexpress
2016-10-28 | GSE84882 | GEO
2016-10-28 | GSE80398 | GEO