Proteomics

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Identification of excavatolide B targets in RAW264.7 macrophages by chemoproteomics


ABSTRACT: The fused macrocycles and multiple stereocenters of briarane-type diterpenoids pose a major challenge to total synthesis and efforts to characterize their biological activities. Harnessing a scalable source of excavatolide B (excB) from cultured corals, we developed two structurally distinct clickable excB probes that retained the anti-inflammatory biological activities of excB. Here we used these probes in a chemoproteomics strategy to the cellular target(s) of excB in mammalian cells.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

DISEASE(S): Disease Free

SUBMITTER: Mingzi Zhang  

LAB HEAD: Mingzi M Zhang

PROVIDER: PXD042748 | Pride | 2023-07-20

REPOSITORIES: Pride

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