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Intracellular zinc chaperones in plants

ABSTRACT: Metal availability is tightly regulated within cells through metal homeostasis to prevent cytotoxicity from excess and protein inactivity from deficiency. Limited bioavailability of essential metals in soil can negatively impact crop health and yield. A plant zinc (Zn)-homeostatic mechanism involving Zn transferases has been discovered, which plays a crucial role in delivering zinc to vital zinc-dependent proteins, particularly under zinc limitation. This study focused on investigating the effects of loss of AtZNG1 (AT1G26520), a key component of this mechanism, on growth, transcriptome, and proteome in Arabidopsis thaliana during zinc deficiency. The findings from multi-omics analyses highlight the broad consequences of this mechanism's disruption. The goal of this experiment wass to identify differentially abundant proteins in A.thaliana root tissue upon Zn withdrawal, in S999 zng1 mutant cells and wild-type control.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Root

SUBMITTER: Paolo Cifani

LAB HEAD: Paolo Cifani

PROVIDER: PXD042911 | Pride | 2025-08-21

REPOSITORIES: Pride

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Source:

			Action	DRS
	221121_PC_FTFT60K_TMT_200.meth	Other
	221212_LZ_proj72_TMT16_1.raw	Raw
	221212_LZ_proj72_TMT16_1_20221220171334.raw	Raw
	221212_LZ_proj72_TMT16_2.raw	Raw
	221212_LZ_proj72_TMT16_2_20221220200533.raw	Raw

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Project description:Zinc is an essential nutrient because of its role in catalysis and in stabilizing protein structure, but excess zinc can also be deleterious. Four nutritional zinc states have been identified in the alga Chlamydomonas reinhardtii: zinc toxic, zinc replete, zinc deficient and zinc limited. Growth is inhibited in zinc-limited and zinc toxic cells relative to zinc-replete cells, while zinc-deficiency is visually asymptomatic but distinguished by the accumulation of transcripts encoding ZIP family transporters. To identify targets of zinc deficiency and mechanisms of zinc acclimation, we used RNA-seq to probe zinc nutrition responsive changes in gene expression. We identified a subset of genes encoding zinc-handling components, including ZIP family transporters and candidate zinc chaperones. In addition, we noted an impact on two other regulatory pathways, the carbon concentrating mechanism (CCM) and the nutritional copper regulon. Targets of transcription factor Ccm1 and various CAH genes are up-regulated in zinc-deficiency, as a likely consequence of reduced carbonic anhydrase activity, which is validated by mass spectrometry and immunoblot analysis of Cah1, Cah3 and Cah4. Chlamydomonas is therefore not able to grow photoautotrophically in air in zinc limiting conditions, but supplementation with 1% CO2 restores growth to wild-type rates, suggesting that the inability to maintain CCM is a major consequence of zinc limitation. Surprisingly, we noted also that the Crr1 regulon, which responds to Cu limitation, is also turned on in zinc deficiency, and in fact, Crr1 is required for growth in zinc-limiting conditions. Zinc deficient cells are functionally copper deficient, as evidenced by reduced plastocyanin abundance, even though they hyperaccumulate copper up to 50-fold over normal levels. We suggest that zinc-deficient cells sequester Cu in a bio-unavailable form, perhaps to prevent mis-metallation of critical zinc sites. Zn-limited wild-type cells were generated by transfer of cells from the first round of growth in medium with no supplemental Zn into TAP medium supplemented or not with 2.5 ÂµM Zn-EDTA The control samples for this study are represented in GSE25622

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Intracellular zinc chaperones in plants

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