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Proteomic and phosphoproteomic profiling of mouse embryonic fibroblasts

ABSTRACT: Fibroblasts are the most common cell type in stroma and function in the support and repair of most tissues. Mouse embryonic fibroblasts (MEFs) are amenable to isolation and rapid growth in culture. To facilitate their use as a research tool, we have performed a comprehensive proteomic and phosphoproteomic characterisation of wildtype primary MEFs from C57BL/6 mice. EIF2/4 and MTOR signalling pathways were enriched in both the proteome and phosphoproteome, along with extracellular matrix (ECM) and cytoskeleton associated pathways. Consistent with this, kinase enrichment analysis identified activation of P38A, P90RSK, P70RSK, and MTOR. Cell surface markers and matrisome proteins were also annotated.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryonic Fibroblast, Fibroblast

SUBMITTER: Nicole Verrills

LAB HEAD: Nicole Verrills

PROVIDER: PXD043244 | Pride | 2023-10-20

REPOSITORIES: Pride

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Proteomic and phosphoproteomic characterisation of primary mouse embryonic fibroblasts.

Chen Yanfang Y Roselli Severine S Panicker Nikita N Brzozowski Joshua S JS Skerrett-Byrne David A DA Murray Heather C HC Verrills Nicole M NM

Proteomics 20231017 7

Fibroblasts are the most common cell type in stroma and function in the support and repair of most tissues. Mouse embryonic fibroblasts (MEFs) are amenable to isolation and rapid growth in culture. MEFs are therefore widely used as a standard model for functional characterisation of gene knockouts, and can also be used in co-cultures, commonly to support embryonic stem cell cultures. To facilitate their use as a research tool, we have performed a comprehensive proteomic and phosphoproteomic char ...[more]

PMID: 37849217

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