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Neonatal and adult platelet proteomics

ABSTRACT: We isolated resting platelets from full term umbilical cord blood and adult peripheral blood. We used DIA mass spectrometry to analyze the whole proteome and phosphoproteome from this platelet samples.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Platelet

SUBMITTER: Hossein Fazelinia

LAB HEAD: Christopher S Thom MD, PhD

PROVIDER: PXD043330 | Pride | 2024-05-01

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20221027_104942_CT1703wp_dDIA.sne	Other
	20221101_105352_CT1703i_mergedSL.sne	Other
	metadata_phos.csv	Csv
	metadata_wholeproteome.csv	Csv
	x221010_CT1703wp_DIA_01.raw	Raw

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Publications

Quantitative label-free mass spectrometry reveals content and signaling differences between neonatal and adult platelets.

Thom Christopher S CS Davenport Patricia P Fazelinia Hossein H Soule-Albridge Erin E Liu Zhi-Jian ZJ Zhang Haorui H Feldman Henry A HA Ding Hua H Roof Jennifer J Spruce Lynn A LA Ischiropoulos Harry H Sola-Visner Martha M

Journal of thrombosis and haemostasis : JTH 20231230 5

<h4>Background</h4>Recent clinical studies have shown that transfusions of adult platelets increase morbidity and mortality in preterm infants. Neonatal platelets are hyporesponsive to agonist stimulation, and emerging evidence suggests developmental differences in platelet immune functions.<h4>Objectives</h4>This study was designed to compare the proteome and phosphoproteome of resting adult and neonatal platelets.<h4>Methods</h4>We isolated resting umbilical cord blood-derived platelets from h ...[more]

PMID: 38160730

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