Proteomic profiling of oleamide-mediated polarization in a primary human monocyte-derived tumor-associated macrophages model: a functional analysis
Ontology highlight
ABSTRACT:
INSTRUMENT(S):
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Macrophage
SUBMITTER: 
Watunyoo Buakaew
LAB HEAD: Kanchana Usuwanthim
PROVIDER: PXD043461 | Pride | 2024-08-25
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Donor-AB.xlsx | Xlsx | |||
| Donor-O2.xlsx | Xlsx | |||
| T1_01.raw | Raw | |||
| T1_02.raw | Raw | |||
| T1_03.raw | Raw |
Items per page: 5 1 - 5 of 20 |
Similar Datasets
Project description:Identification of common Leishmania antigen proteins is important for practical purposes ranging from diagnostic to the development of a vaccine for the prevention of leishmaniasis. Moreover, we need to establish the potential drug targets that interrupt the replication and transmission of the pathogen to promote drug discovery. Therefore, the objective of this work was to provide the public proteome database which would be useful for the establishment of novel protein antigenicity for therapeutic purposes. This information would introduce the alternative protein targets for Leishmaniasis, allowing the development of therapeutic strategies for the disease.
2022-05-20 | PXD026304 | Pride
Project description:The co-cultivation investigation showed that A. cornea TBRC 12900 co-cultivated with P. mangrovi TBRC-BCC 42794 showed a high level of A. cornea mycelial growth. Proteomics analysis was employed to reveal predicted biological activities involved in the mutualistic relationship between the two strains during mycelial growth.
2022-10-27 | PXD035814 | Pride
Project description:Nonalcoholic fatty liver disease (NAFLD) is associated with an increased risk of cardiovascular disease (CVD). Liver is the major source of the circulatory proteins and alterations in plasma proteome have been implicated in atherosclerosis. We used a recently developed 2H2O-metabolic labeling method in combination with the label-free quantification to evaluate the effect of a Western diet (WD) on the dynamics of plasma proteins in LDL receptor-deficient (LDLR-/-) mice, a model of NAFLD and atherosclerosis. There were no significant changes in the expression level of total proteins due to the WD with a bulk protein mean half-life of 18.0±15.1 hours in control and 16.7±11.1 hours in WD groups. WD led to pro-inflammatory distribution of circulatory proteins analyzed in apoB-depleted plasma attributed to their increased production. The fractional catabolic rates of fast-turnover proteins with stress-response, lipid metabolism and transport functions were significantly increased with WD (P<0.05). The pathway analyses revealed that alterations in plasma proteome dynamics were related to the suppression of hepatic PPARα, which was confirmed based on reduced gene and protein expression of PPARα on WD. These changes were associated with ~4 fold increase (P<0.0001) in pro-inflammatory property of apoB-depleted plasma. In conclusion, the proteome dynamics method reveals pro-inflammatory remodeling of plasma proteome relevant to liver disease. As in vivo metrics of liver function, this approach can be used to test therapies in NAFLD and other diseases
2016-06-06 | PXD004270 | Pride
Project description:Corneal wound healing involves complex cellular processes, including keratocyte cell death, which plays a pivotal role in initiating tissue repair. Recent studies suggest that pyroptosis, characterized by the release of pro-inflammatory factors, occurs during tissue repair which may also contribute to the inflammatory phase of corneal wound healing. In this study, we investigated the nature of cell death in human keratocytes treated with nigericin, a known pyroptosis inducer. Surprisingly, nigericin triggered a unique form of non-inflammatory cell death marked by extensive vacuolation, resembling paraptosis. Proteomic analysis revealed that our human keratocytes lack key components of the canonical pyroptosis pathway, including NLRP3, ASC, and caspase-1, potentially explaining the absence of pyroptosis in these cells. This process was further confirmed by the absence of caspase-3 activation and lack of ER stress, alongside increased autophagic marker LC3-II accumulation. These findings suggest that nigericin triggers a paraptosis-like cell death in keratocytes, rather than pyroptosis, highlighting an alternative mechanism of cell death in corneal wound healing that warrants further exploration.
2025-06-30 | PXD058468 | Pride
Project description:Tuberculosis, caused by Mycobacterium tuberculosis, still remains a major global health problem. The main obstacle in eradicating this disease is the ability of this pathogen to remain dormant in macrophages, and to get reactivated later under immuno-compromised conditions. The physiology of hypoxic nonreplicating M. tuberculosis is well studied using many in vitro dormancy models. However, the physiological changes that take place during the shift from dormancy to aerobic growth (reactivation) have rarely been subjected to a detailed investigation. In this study, we developed an in vitro reactivation system by re-aerating bacteria that were made dormant employing Wayne’s dormancy model, and compared the proteome profiles of dormant and reactivated bacteria using label-free one-dimensional LC/MS/MS analysis.
2015-06-01 | PXD001158 | Pride
Project description:Herein we demonstrate the efficacy of an unbiased proteomics screening approach for studying protein expression changes in the KC-Tie2 psoriasis mouse model, identifying multiple protein expression changes in the mouse and validating these changes in human psoriasis. KC-Tie2 mouse skin samples (n=3) were compared with littermate controls (n=3) using gel-based fractionation followed by label-free protein expression analysis. 5482 peptides mapping to 1281 proteins were identified and quantitated: 105 proteins exhibited fold-changes ≥2.0 including: stefin A1 (average fold change of 342.4 and an average P = 0.0082; cystatin A, human orthologue); slc25a5 (average fold change of 46.2 and an average P = 0.0318); serpinb3b (average fold change of 35.6 and an average P = 0.0345; serpinB1, human orthologue); and kallikrein related peptidase 6 (average fold change of 4.7 and an average P = 0.2474; KLK6). We independently confirmed mouse gene expression-based increases of selected genes including serpinb3b (17.4-fold, P < 0.0001), KLK6 (9.0-fold, P = 0.002), stefin A1 (7.3-fold; P < 0.001) and slc25A5 (1.5-fold; P = 0.05) using qRT-PCR on a second cohort of animals (n=8). Parallel LC/MS/MS analyses on these same samples verified protein-level increases of 1.3-fold (slc25a5; P < 0.05), 29,000-fold (stefinA1; P < 0.01), 322-fold (KLK6; P < 0.0001) between KC-Tie2 and control mice. To underscore the utility and translatability of our combined approach, we analyzed gene and protein expression levels in psoriasis patient skin and primary keratinocytes vs. healthy controls. Increases in gene expression for slc25a5 (1.8-fold), cystatin A (3.0-fold), KLK6 (5.8-fold) and serpinB1 (76-fold; all P < 0.05) were observed between healthy controls and involved lesional psoriasis skin and primary psoriasis keratinocytes. Moreover slc25a5, cystatin A, KLK6 and serpinB1 protein were all increased in lesional psoriasis skin compared to normal skin. These results highlight the usefulness of preclinical disease models using readily-available mouse skin and demonstrate the utility of proteomic approaches for identifying novel peptides/proteins that are differentially regulated in psoriasis that could serve as sources of auto-antigens or provide novel therapeutic targets for the development of new anti-psoriatic treatments.
2014-10-30 | PXD001387 | Pride
Project description:A mutation in the proteasomal lid subunit Rpn8a alters proteasome composition and exerts a beneficial effect on photosynthesis. The effect is particularly visible in a double mutant between rpn8a and the plastid protein import receptor Toc159 (ppi2xrpn8a) but also in an rpn8a single mutant that shows higher PSII efficiency and lower non-photochemical quenching. The double mutant shows elevated accumulation of pigments, higher levels of thylakoid membrane proteins and higher PSII quantum yield compared to the ppi2 single mutant. While proplastid-like pro-thylakoid structures are characteristic for ppi2, stacked thylakoids are frequently observed in the double mutant. The rpn8a mutation is accompanied by higher 20S and lower 26S proteasome abundance and increased expression levels of proteolytic stress markers. Unlike known retrograde signaling chains, there is no effect on the transcription of selected photosynthesis associated nuclear encoded gens (PhanGs). Since plastid precursor protein abundance is elevated in the double mutant it is conceivable that the changes in proteasome composition affect precursor stability. We propose a model in which mild proteasome impairment shifts the equilibrium between precursor import and degradation towards import, which is especially effective in plastid protein import mutants but also detectable in the wildtype background, albeit to a much weaker extent.
2020-05-27 | PXD014531 | Pride
Project description:To decipher the protective mechanisms underlying microglia specific heterozygous TAK1 deficiency in rd10 mouse model of retinitis pigmentosa, we analyzed the retinal proteomic profiles of normal C57BL/6J mice, tamoxifen-treated Cx3cr1CreER/+;Tak1fl/+, vehicle-treated rd10;Cx3cr1CreER/+;Tak1fl/+, and tamoxifen-treated rd10;Cx3cr1CreER/+;Tak1fl/+ mice using a data-independent acquisition-based mass spectrometry (DIA).
2025-04-17 | PXD061300 | Pride
Project description:In this study, a total of 10 patients who received rt-PA were enrolled. Plasma samples were collected before and 1 hour after the rt-PA infusion for proteomic analysis. Blood sample collection has been approved by Tianjin Medical University General Hospital Ethics Committee, and collection was conducted according to the Declaration of Helsinki. Informed consent was obtained from all participants, and all experiments were conducted in accordance with Chinese laws and institutional guidelines.
2024-07-12 | PXD046738 | Pride
Project description:In this study, a total of 10 patients who received rt-PA were enrolled. Plasma samples were collected before and 1 hour after the rt-PA infusion for proteomic analysis. Blood sample collection has been approved by Tianjin Medical University General Hospital Ethics Committee, and collection was conducted according to the Declaration of Helsinki. Informed consent was obtained from all participants, and all experiments were conducted in accordance with Chinese laws and institutional guidelines.
2024-07-12 | PXD046676 | Pride