Proteomics

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Mouse and Human Conjunctiva Organoids to Study Ocular Surface Homeostasis and Disease


ABSTRACT: The conjunctival epithelium covering the eye consists of two main differentiated cell types: mucus-producing Goblet cells and ‘keratinocytes’ of unknown function. Here, we describe long-term expanding organoids representing human conjunctiva. To check if interleukins may boost secretome expression, we stimulated the organoid cells with pleiotropic anti-inflammatory cytokines interleukin-4 and IL-13. Secretions were identified by shotgun MS to quantitatively enriched secreted proteins.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Ziliang Ma  

LAB HEAD: Wei Wu

PROVIDER: PXD043550 | Pride | 2024-01-11

REPOSITORIES: Pride

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