Dataset Information

Assessment of the effect of 21-day head-down bed rest on the cardiovascular system by blood protein composition, including markers sST2, NT-proBNP and D-dimer

ABSTRACT: Head-down bed rest (HDBR) is one of the models of the physiological effects of weightlessness used, among other things, to assess the effect of hypokinesia on the physiological systems of the human body and, first of all, on the cardiovascular system. The aim of the work was to study the effect of 21-day HDBR factors on the cardiovascular system based on blood proteomic profile data and myocardial stress biomarkers sST2, NT-proBNP, and the thrombogenesis risk marker D-dimer. It was revealed that HDBR conditions led to an increase in the level of proteins of the complement and the coagulation cascade systems, platelet degranulation, fibrinolysis, acute phase proteins, post-translational modification of proteins, retinol-binding protein 4 (RBP4), apolipoprotein B, S100A8, associated with cardiovascular diseases, and other proteins that affect the functions of endothelial cells. It has been shown that the level of proteins in the blood involved in the remodeling of the cytoskeleton, oxygen transport, heme catabolism, etc. decreased during HDBR. A significant decrease in the levels of NT-proBNP and sST2 by the 21st day of HDBR and a rapid recovery of their levels already on the first day of the rehabilitation period was established, which indicated the restoration of hemodynamic parameters of the heart function. The levels of D-dimer in different periods of the experiment and after its completion (on the 14th day) did not differ from the background values, which indicated the absence of the risk of thrombosis during this exposure and within 14 days after its completion.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Alexander Brzhozovskiy

LAB HEAD: Irina Larina

PROVIDER: PXD043604 | Pride | 2026-07-01

REPOSITORIES: Pride

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Assessment of the effect of 21-day head-down bed rest on the cardiovascular system by blood protein composition.

Kashirina Daria N DN Pastushkova Ludmila Kh LK Goncharova Anna G AG Larina Irina M IM

Frontiers in physiology 20240620

Head-down bed rest (HDBR) is one of the models of the physiological effects of weightlessness used, among other things, to assess the effect of hypokinesia on the physiological systems of the human body and, first of all, on the cardiovascular system. The aim of the work was to study the effect of 21 days of HDBR factors on the cardiovascular system based on blood proteomic profile data. It was revealed that HDBR conditions led to an increase in the levels of proteins of the complement and the c ...[more]

PMID: 38966226

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Project description:Eight male C57BL/6 mice were used in this study. Four (denoted by My*, V*, E*, S*) of the mice were from the same litter of one biological mother (207H), and the other four mice (denoted by J, Ms, N, U) were from the same litter of another biological mother (189C). To explore the potential maternal and co-housing effects, the eight mice were housed in three different individual ventilated cages as follows: My* and V* (littermates of mother mouse 207H) were housed in cage 1; N and U (littermates of mother mouse 189C) were housed in cage 3; E* and S* (littermates of mother mouse 207H), and J and Ms (littermates of mother mouse 189C) were co-housed in cage 2. All mice were housed in a 12-hr light/dark cycle and fed irra- diated water and standard food after weaning at the age of 21 days. Mice were obtained from the Animal and Plant Care Facility of The Hong Kong University of Science and Technology, and were bred at the core facility. All experimental procedures involving animals were conducted in compliance with the Animal User Manual and approval was obtained from the Animal Ethics Committee of The Hong Kong University of Science and Technology. Fecal samples of each mouse were independently collected on the 21st, 22nd, 23rd, 29th, 30th, and 31st day after birth. For individual mouse, fecal samples from the 21st and 22nd day (--3 weeks of age, 1st and 2nd day after weaning), and samples from the 29th and 30th day (--4 weeks of age, 9th and 10th day after weaning), were pooled and subjected to metagenomic sequencing, respectively; samples from the 23rd day (--3 weeks of age, 3rd day after weaning), and the 31 days (--4 weeks of age, 11th day after weaning) were subjected to metaproteomic analysis separately. The 16 metaproteomic samples were analyzed by metaSpectraST, which indicated a clear gut microbiome changes throughout the short period after weaning, which was possibly associated with the dietary shift from milk to solid food, while no distinctive changes were observed by metagenomic analysis.

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	HDBR_1.1.d.rar	Other
	HDBR_1.2.d.rar	Other
	HDBR_2.1.d.rar	Other
	HDBR_2.2.d.rar	Other
	HDBR_3.1.d.rar	Other