An oligosaccharyltransferase from Leishmania donovani increases the N-glycan occupancy on plant-produced IgG1
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ABSTRACT: The monoclonal antibodies rituximab and cetuximab were transiently expressed in leaves of Nicotiana benthamiana plants and purified by affinity chromatography using protein A. Purified antibodies were analysed by intact MS. Purified antibodies were also subjected to trypsin digestion followed by LC-ESI-MS analysis of the glycopeptide carrying the N-glycsylation site Asn297 using the LC-OTMS, Orbitrap Exploris 480 from Thermo Scientific.
INSTRUMENT(S): Orbitrap Exploris 480
ORGANISM(S): Nicotiana Benthamiana
TISSUE(S): Plant Cell, Leaf
SUBMITTER: Richard Strasser
LAB HEAD: Richard Strasser
PROVIDER: PXD043623 | Pride | 2023-07-31
REPOSITORIES: Pride
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