Dataset Information

High-Intensity Comparative and Quantitative Proteomics of Histamine-Forming and Other Biogenic Amine-Producing Bacteria Present in Seafood

ABSTRACT: This article describes a shotgun quantitative proteomics strategy to evaluate and compare foodborne strains of bacteria that produce biogenic amines.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Providencia Stuartii Klebsiella Oxytoca Nbrc 105695 Klebsiella Pneumoniae Kp-1 Proteus Mirabilis Bb2000 Proteus Vulgaris Providencia Rettgeri Enterobacter Sp. T3 Hafnia Alvei

SUBMITTER: Mónica Carrera

LAB HEAD: Monica Carrera

PROVIDER: PXD043997 | Pride | 2025-05-06

REPOSITORIES: Pride

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Publications

High-Resolution Comparative and Quantitative Proteomics of Biogenic-Amine-Producing Bacteria and Virulence Factors Present in Seafood.

Abril Ana G AG Calo-Mata Pilar P Villa Tomás G TG Böhme Karola K Barros-Velázquez Jorge J Sánchez-Pérez Ángeles Á Pazos Manuel M Carrera Mónica M

Journal of agricultural and food chemistry 20240216 8

The presence of biogenic amines (histamine, tyramine, putrescine, and cadaverine) in seafood is a significant concern for food safety. This review describes for the first time a shotgun quantitative proteomics strategy to evaluate and compare foodborne strains of bacteria that produce biogenic amines in seafoods. This approach recognized 35,621 peptide spectrum matches, belonging to 20,792 peptides, and 4621 proteins. It allowed the determination of functional pathways and the classification of ...[more]

PMID: 38364257

Similar Datasets

Project description:Biogenic amine-producing bacteria are responsible for the production of basic nitrogenous compounds, such as histamine, cadaverine, tyramine and putrescine, after foods spoil due to microorganisms. In the present work, we applied a shotgun proteomics approach to quickly and easily characterize 15 different foodborne strains of biogenic amine-producing bacteria. A total of 10673 peptide spectrum matches (PSMs) belonging to 4081 nonredundant peptides and corresponding to 1811 annotated proteins were identified. With the results, relevant functional pathways were determined and the strains were differentiated into different Euclidean hierarchical clusters. Moreover, a predicted protein‒protein interaction network of biogenic amine foodborne strains was created. The whole confidence network contains 260 nodes and 1973 interactions. Most of the identified proteins were related to pathways and networks of energy, putrescine metabolism and host‒virus interaction. In addition, a total of 556 nonredundant peptides were identified as virulence factors, and most of these peptides corresponded to functions such as toxins, antimicrobial compound production, antimicrobial resistance, additional resistances and tolerances, host colonization and immune evasion, ABC transporters, phage proteins, and alternative virulence factors and proteins involved in horizontal transfer. Potential species-specific peptide biomarkers were screened. Thus, 77 species-specific peptide biomarkers belonging to 64 different proteins were proposed to identify 10 species (Enterobacter aerogenes, Enterobacter cloacae, Hafnia alvei, Klebsiella oxytoca, Morganella morganii, Proteus mirabilis, Proteus penneri, Proteus vulgaris, Raoutella planticola, Stenotrophomonas maltophilia). All of these results constitute the first major dataset of peptides and proteins of seafood biogenic amine-producing strains. This repository may be useful for further studies, for the development of new therapeutic treatments for food intoxication and for tracking microbial sources in foodstuffs.

			Action	DRS
	Histaminicas_Vigo_LFQ_b.mzML	Mzml
	Histaminicas_Vigo_LFQ_b.mzid.gz	Mzid
	MCMJMM170450_10.raw	Raw
	MCMJMM170450_11.raw	Raw
	MCMJMM170450_11_b.raw	Raw