Dataset Information

Peptide profiling of sheep milk kefir through peptidomic analysis at different fermentation times

ABSTRACT: In this study, we applied a proteomics strategy to identify peptides present in sheep milk kefir fermented at different times. We aimed to understand changes in the digestion pattern of milk proteins as well as to identify potential bioactive peptides.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Ovis Aries

TISSUE(S): Milk

SUBMITTER: Sevim Dalabasmaz

LAB HEAD: Manuel J. Rodríguez-Ortega

PROVIDER: PXD044067 | Pride | 2023-08-20

REPOSITORIES: Pride

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Publications

Identification of Potential Bioactive Peptides in Sheep Milk Kefir through Peptidomic Analysis at Different Fermentation Times.

Dalabasmaz Sevim S de la Torre Esther Prados EP Gensberger-Reigl Sabrina S Pischetsrieder Monika M Rodríguez-Ortega Manuel J MJ

Foods (Basel, Switzerland) 20230807 15

Sheep farming is an important socioeconomic activity in most Mediterranean countries, particularly Spain, where it contributes added value to rural areas. Sheep milk is used in Spain mainly for making cheese, but it can be used also for making other dairy products, such as the lactic-alcoholic fermentation product known as kefir. Dairy products have health benefits because, among other reasons, they contain molecules with biological activity. In this work, we performed a proteomics strategy to i ...[more]

PMID: 37569243

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Project description:Microbial communities often undergo intricate compositional changes yet also maintain stable coexistence of diverse species. The mechanisms underlying long-term coexistence remain unclear, as system-wide studies have been largely limited to engineered communities, ex situ adapted cultures, or synthetic assemblies. Here we show how kefir, a natural milk-fermenting community of prokaryotes and yeasts, realises stable coexistence through spatiotemporal orchestration of species and metabolite dynamics. During milk fermentation, kefir grains (a polysaccharide matrix synthesized by kefir microbes) grow in mass but remain unchanged in composition. In contrast, the milk is colonized in a sequential manner in which early members open metabolic niches for followers. Through metabolomics and large-scale mapping of inter-species interactions, we show how microbes poorly suited for milk survive in, and even dominate the community, through metabolic cooperation and uneven partitioning between grain and milk. Overall, our findings reveal how inter-species interactions partitioned in space and time lead to stable coexistence. Linked metabolomics studies:<a href='https://www.ebi.ac.uk/metabolights/MTBLS1823' rel='noopener noreferrer' target='_blank'>MTBLS1823</a> Spent-medium assay, measured untargeted (FIA-qTOF) and targeted (HILIC, LC-MS)<a href='https://www.ebi.ac.uk/metabolights/MTBLS1830' rel='noopener noreferrer' target='_blank'>MTBLS1830</a> Interaction between the kefir isolates Lactococcus lactis and Acetobacter fabarum (GC-MS) Linked cross omic data sets:Genomes of isolated kefir species are available in the NCBI database under the accession: PRJNA375758 (bioproject ID: 375758).Metatranscriptomic sequencing data associated with this study are available in the European Nucleotide Archive (ENA): accession number <a href='https://www.ebi.ac.uk/ena/browser/view/PRJEB37001' rel='noopener noreferrer' target='_blank'>PRJEB37001</a>.Genome-scale metabolic models for kefir bacteria can be found at github.com/cdanielmachado/kefir_models.

Dataset Information

Peptide profiling of sheep milk kefir through peptidomic analysis at different fermentation times

Publications

Identification of Potential Bioactive Peptides in Sheep Milk Kefir through Peptidomic Analysis at Different Fermentation Times.

Similar Datasets

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