Proteomics

Dataset Information

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Kidney tissue pipeline for multi-omics and histological analysis - LCMSMS of porcine (pig) kidney biopsies


ABSTRACT: In this study, our primary objective was to establish a streamlined workflow for the collection of kidney biopsies obtained during clinical procedures. Our aim was to ensure that this workflow integrates into the daily routines while also preserving the integrity of the precious samples for subsequent analysis using advanced techniques. To achieve this, we modified a robust protocol for dissociating single nuclei from variously stored kidney tissue samples, including snap frozen, RNAlater, and CellCover preserved specimens. These single nucleus (sn) suspensions were then processed using the widely recognized Chromium 10X Genomics platform. We conducted snRNAseq analysis on the resulting datasets from each storage method to identify any potential variations in transcriptomic profiles. Furthermore, we assessed the suitability of the preservation media for additional analyses such as proteomics, metabolomics, and the preservation of tissue architecture for immunofluorescence staining. This evaluation aimed to determine if the chosen preservation methods effectively maintained the necessary quality and characteristics of the samples for diverse analytical approaches. Overall, our study aimed to develop an efficient and adaptable workflow for the collection and preservation of clinically obtained kidney biopsies, enabling comprehensive analysis using cutting-edge techniques while ensuring the preservation of valuable samples.

INSTRUMENT(S):

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Kidney

SUBMITTER: Moritz Lassé  

LAB HEAD: Markus M. Rinschen

PROVIDER: PXD044127 | Pride | 2025-06-27

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
210604_CK_ML_0002_S01.raw Raw
210604_CK_ML_0002_S02.raw Raw
210604_CK_ML_0002_S03.raw Raw
210604_CK_ML_0002_S04.raw Raw
210604_CK_ML_0002_S05.raw Raw
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