Dataset Information

Uterine fluid extracellular vesciles proteome in bovine clinical and subclinical endometritis

ABSTRACT: Uterine fluid (UF) samples were acquired from healthy cows, cows with clinical (CLE) and subclinical endometritis (SE). The extracellular vesicles (EVs) were isolated using size exclusion chromatography-based method. The enrichment of UF-EVs were confirmed with mass-spectrometry of a sample before and after UF-EV isolation, transmission electron microscopy and nanoparticle tracking analysis. We used LC/MS-MS based quantative shotgun proteomics to compare UF-EV proteomes in healthy cows, cows with SE or CLE acquired at 28 to 35 days postpartum (n = 3 per group).

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Uterine Fluid

DISEASE(S): Endometritis

SUBMITTER: Johanna Piibor

LAB HEAD: Alireza Fazeli

PROVIDER: PXD044219 | Pride | 2026-04-27

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	230308_24_Orbi3_MS_SER_G60_T85_EMY_Fazeli-Piibor_SE1.HCD.FTMS.peak.apl	Other
	230308_24_Orbi3_MS_SER_G60_T85_EMY_Fazeli-Piibor_SE1.HCD.FTMS.secpep.sil0.apl	Other
	230308_24_Orbi3_MS_SER_G60_T85_EMY_Fazeli-Piibor_SE1.HCD.FTMS.sil0.apl	Other
	230308_24_Orbi3_MS_SER_G60_T85_EMY_Fazeli-Piibor_SE1.raw	Raw
	230308_26_Orbi3_MS_SER_G60_T85_EMY_Fazeli-Piibor_CLE3.HCD.FTMS.peak.apl	Other

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Publications

Investigation of Uterine Fluid Extracellular Vesicles' Proteomic Profiles Provides Novel Diagnostic Biomarkers of Bovine Endometritis.

Piibor Johanna J Waldmann Andres A Prasadani Madhusha M Kavak Ants A Andronowska Aneta A Klein Claudia C Kodithuwakku Suranga S Fazeli Alireza A

Biomolecules 20240525 6

Cow uterine infections pose a challenge in dairy farming, resulting in reproductive disorders. Uterine fluid extracellular vesicles (UF-EVs) play a key role in cell-to-cell communication in the uterus, potentially holding the signs of aetiology for endometritis. We used mass spectrometry-based quantitative shotgun proteomics to compare UF-EV proteomic profiles in healthy cows (H), cows with subclinical (SE) or clinical endometritis (CLE) sampled at 28-35 days postpartum. Functional analysis was ...[more]

PMID: 38927030

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