Uterine fluid extracellular vesciles proteome in bovine clinical and subclinical endometritis

ABSTRACT: Uterine fluid (UF) samples were acquired from healthy cows, cows with clinical (CLE) and subclinical endometritis (SE). The extracellular vesicles (EVs) were isolated using size exclusion chromatography-based method. The enrichment of UF-EVs were confirmed with mass-spectrometry of a sample before and after UF-EV isolation, transmission electron microscopy and nanoparticle tracking analysis. We used LC/MS-MS based quantative shotgun proteomics to compare UF-EV proteomes in healthy cows, cows with SE or CLE acquired at 28 to 35 days postpartum (n = 3 per group).

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Uterine Fluid

DISEASE(S): Endometritis

SUBMITTER: Johanna Piibor  

LAB HEAD: Alireza Fazeli

PROVIDER: PXD044219 | Pride | 2026-04-27

REPOSITORIES: Pride