Proteomics

Dataset Information

0

Interactome analysis of PHF2 using LC-MS combined with an immunoprecipitation assay


ABSTRACT: PHF2 demethylates lysine 9 in histone 3 and acts as a tumor suppressor. In liver pathogenesis, PHF2 has dual effects. PHF2 induces simple hepato-steatosis by epigenetically coactivating ChREBP. When lipid accumulation goes serious, it protects the liver from fibrogenesis by facilitating Nrf2 activity, a major transcription factor in the defense against oxidative stress during non-alcoholic fatty liver disease. However, little is known regarding the functions of PHF2 in liver cancer cells. Therefore, to investigate the possible roles of PHF2 and find new interacting proteins, we performed an LC-MS/MS assay combined with an immunoprecipitation assay.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Liver, Cell Culture

DISEASE(S): Hepatocellular Carcinoma

SUBMITTER: Do-Won Jeong  

LAB HEAD: Yang-Sook Chun

PROVIDER: PXD044277 | Pride | 2023-10-18

REPOSITORIES: Pride

Similar Datasets

2020-10-19 | PXD019137 | Pride
2023-03-11 | PXD037453 | Pride
2020-01-24 | GSE139937 | GEO
2017-03-21 | GSE96820 | GEO
2023-04-22 | GSE143357 | GEO
2022-04-15 | GSE159518 | GEO
2012-08-31 | E-GEOD-39977 | biostudies-arrayexpress
2012-09-01 | E-GEOD-34641 | biostudies-arrayexpress
2022-11-15 | GSE217982 | GEO
2022-11-15 | GSE217937 | GEO