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Impact of mutations on the plant-based production of recombinant SARS-CoV-2 RBD

ABSTRACT: Recombinant RBD variants from SARS-CoV-2 spike were produced by transient expression in Nicotiana benthamiana plants and purified. The purified proteins were digested with proteases and glycopeptides were analysed by MS to identify the N-glycan structures.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Nicotiana Benthamiana

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Richard Strasser

LAB HEAD: Richard Strasser

PROVIDER: PXD044462 | Pride | 2024-01-26

REPOSITORIES: Pride

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Impact of mutations on the plant-based production of recombinant SARS-CoV-2 RBDs.

Ruocco Valentina V Vavra Ulrike U König-Beihammer Julia J Bolaños Martínez Omayra C OC Kallolimath Somanath S Maresch Daniel D Grünwald-Gruber Clemens C Strasser Richard R

Frontiers in plant science 20231006

Subunit vaccines based on recombinant viral antigens are valuable interventions to fight existing and evolving viruses and can be produced at large-scale in plant-based expression systems. The recombinant viral antigens are often derived from glycosylated envelope proteins of the virus and glycosylation plays an important role for the immunogenicity by shielding protein epitopes. The receptor-binding domain (RBD) of the SARS-CoV-2 spike is a principal target for vaccine development and has been ...[more]

PMID: 37868317

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