Dataset Information

DHFR2 Proteomic analysis - LC MS/MS NICB DCU

ABSTRACT: LC-MS/MS shotgun proteomic analysis looking at DHFR2 in mammalian cell lines.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

SUBMITTER: Michael Henry

LAB HEAD: Michael Henry

PROVIDER: PXD044513 | Pride | 2025-05-06

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	120min_OTIT_HEPG2_DHFR2_KO_1.mgf	Mgf
	120min_OTIT_HEPG2_DHFR2_KO_1.msfView	Msf
	120min_OTIT_HEPG2_DHFR2_KO_1.mzData	Other
	120min_OTIT_HEPG2_DHFR2_KO_1.mzML	Mzml
	120min_OTIT_HEPG2_DHFR2_KO_1.mzid.gz	Mzid

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Publications

DHFR2 RNA directly regulates dihydrofolate reductase and its expression level impacts folate one carbon metabolism.

Drago Paola P Bookey Niamh N Leung Kit-Yi KY Henry Michael M Meleady Paula P Greene Nicholas D E NDE Parle-McDermott Anne A

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20250201 4

Dihydrofolate reductase activity is required in One Carbon Metabolism to ensure that the biologically active form of folate, tetrahydrofolate, is replenished and available as an enzyme cofactor for numerous cellular reactions, including purine and pyrimidine synthesis. Most cellular enzyme activity was thought to arise from the product of the DHFR gene on chromosome 5, with its paralogue DHFR2 (formerly known as DHFRL1; [chromosome 3]), believed to be responsible for mitochondrial dihydrofolate ...[more]

PMID: 39957677

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