Hydrogen Deuterium Exchange of PI4K(530-560) Peptide with Calcineurin

ABSTRACT: we used HDXMS to study if PI4KA(530-560) is able to bind calcineurin. Here we showed a significant protection in deuterium exchange upon binding calcineurin in the wild type state, indicating these proteins are interacting. Using a mutant that should inhibit the interaction (AKASAA), we showed that this mutant does in fact prevent protection by calcineurin.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: John Burke  

LAB HEAD: Dr. John E. Burke

PROVIDER: PXD044636 | Pride | 2024-09-03

REPOSITORIES: Pride