Proteomics

Dataset Information

0

DNA pulldown of PRDM16 promoter and measurement of LC/MS-MS


ABSTRACT: To further investigate the transcription cofactor of Smad3 that regulated the transcription of PRDM16, we conducted DNA pull-down assay. we treated HK-2 cells with TGF-β and extracted the nuclear proteins. And the nuclear proteins were incubated with streptavidin magnetic beads, and biotin-labeled DNA probes which bound to the promoter of PRDM16 specifically. After repeatedly washing, the pull-down proteins were analyzed by LC-MS/MS proteomics.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Wounds And Injuries

SUBMITTER: Ben Tang  

LAB HEAD: Chun Zhang

PROVIDER: PXD044753 | Pride | 2025-07-14

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
YQ-Control-F001664.txt Txt
YQ-Control.raw Raw
YQ-sample-F001665.txt Txt
YQ-sample.raw Raw
checksum.txt Txt
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