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Human liver cell line LO2 LC-MS/MS

ABSTRACT: A hyperlipidaemia model of L02 cells was established using free fatty acids (FFA, oleate and palmitate, 2:1; Sigma-Aldrich, USA) at a final concentration of 1 mM for 24 h. Cells were allowed to attach overnight prior to HTG-medicated serum for 24 h and then exposed to FFA during drug treatment. After treatment, LO2 cells were harvest and stored at -80 ℃, lactylation proteomics was performed by Jingjie PTM BioLabs (Hangzhou, China).

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Li Min

LAB HEAD: Qingyong He

PROVIDER: PXD045069 | Pride | 2024-01-26

REPOSITORIES: Pride

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Publications

Herbal medicine formula Huazhuo Tiaozhi granule ameliorates dyslipidaemia via regulating histone lactylation and miR-155-5p biogenesis.

Yin Xiangjun X Li Min M Wang Yongzhi Y Zhao Guifang G Yang Tao T Zhang Yuqing Y Guo Jianbo J Meng Tiantian T Du Ruolin R Li Honglin H Wang Zhe Z Zhang Jian J He Qingyong Q

Clinical epigenetics 20231102 1

<h4>Background</h4>Huazhuo Tiaozhi granule (HTG) is a herbal medicine formula widely used in clinical practice for hypolipidaemic effects. However, the molecular mechanisms underlying dyslipidaemia treatment have not been well elucidated.<h4>Results</h4>A significant reduction in the levels of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) was observed in the serum of patients with dyslipidaemia after HTG treatment, without disruption in the levels of aspartate transamina ...[more]

PMID: 37919786

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