Human liver cell line LO2 LC-MS/MS

ABSTRACT: A hyperlipidaemia model of L02 cells was established using free fatty acids (FFA, oleate and palmitate, 2:1; Sigma-Aldrich, USA) at a final concentration of 1 mM for 24 h. Cells were allowed to attach overnight prior to HTG-medicated serum for 24 h and then exposed to FFA during drug treatment. After treatment, LO2 cells were harvest and stored at -80 ℃, lactylation proteomics was performed by Jingjie PTM BioLabs (Hangzhou, China).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Li Min  

LAB HEAD: Qingyong He

PROVIDER: PXD045069 | Pride | 2024-01-26

REPOSITORIES: Pride