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The inhibitor protein IF1 from mammalian mitochondria inhibits ATP hydrolysis but not ATP synthesis by the ATP synthase complex

ABSTRACT: The hydrolytic activity of the ATP synthase in bovine mitochondria is inhibited by a protein called IF1, but bovine IF1 has no effect on the synthetic activity of the bovine enzyme in mitochondrial vesicles in the presence of a proton motive force. As part of an investigation into the inhibitory effects on the hydrolytic and synthetic activities of ATP synthase in bovine sub-mitochondrial particles, the absolute quantities of both the ATP synthase complex and the IF1 protein have been measured.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Heart

SUBMITTER: Ian M. Fearnley

LAB HEAD: John E. Walker

PROVIDER: PXD045079 | Pride | 2024-01-29

REPOSITORIES: Pride

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The inhibitor protein IF1 from mammalian mitochondria inhibits ATP hydrolysis but not ATP synthesis by the ATP synthase complex.

Carroll Joe J Watt Ian N IN Wright Charlotte J CJ Ding Shujing S Fearnley Ian M IM Walker John E JE

The Journal of biological chemistry 20240126 3

The hydrolytic activity of the ATP synthase in bovine mitochondria is inhibited by a protein called IF1, but bovine IF1 has no effect on the synthetic activity of the bovine enzyme in mitochondrial vesicles in the presence of a proton motive force. In contrast, it has been suggested based on indirect observations that human IFI inhibits both the hydrolytic and synthetic activities of the human ATP synthase and that the activity of human IF1 is regulate ...[more]

PMID: 38280428

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