O-GlcNAcylation in mitochondrial protein in rat astrocytes

ABSTRACT: To further investigate this idea that O-GlcNAcylation in mitochondria can improve the efficacy and benefit of mitochondrial transfer, we asked what mitochondrial proteins were modified with O-GlcNAc. To identify O-GlcNAcylated proteins and the potential target sites of O-GlcNAcylation, rat astrocytes were stimulated with NAD to induce O-GlcNAcylation in mitochondria, then we isolated mitochondria and extracted O-GlcNAcylated proteins by immunoprecipitation using O-GlcNAc antibody followed by silver staining.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain, Fibroblast

DISEASE(S): Disease Free

SUBMITTER: Kazuhide Hayakawa  

LAB HEAD: Kazuhide Hayakawa

PROVIDER: PXD045316 | Pride | 2023-10-20

REPOSITORIES: Pride