Dataset Information

Low-melting point agarose as embedding medium for MALDI mass spectrometry imaging and laser-capture microdissection-based proteomics

ABSTRACT: We report an investigation of low-temperature embedding media in terms of their mechanical properties and embedding temperature, with the goal of determining a reliable embedding medium for the combination of MALDI MSI of lipids, laser-capture microdissection of tissue regions, and quantitative proteomics. We compared embedded and non-embedded tissues to ensure that the embedding media did not interfere with the analysis.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Francesco Greco

LAB HEAD: Liam Andrew McDonnell

PROVIDER: PXD045349 | Pride | 2024-01-26

REPOSITORIES: Pride

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Publications

Low-melting point agarose as embedding medium for MALDI mass spectrometry imaging and laser-capture microdissection-based proteomics.

Greco Francesco F Pardini Luca Fidia LF Botto Asia A McDonnell Liam Andrew LA

Scientific reports 20231031 1

The combination of MALDI mass spectrometry imaging, laser-capture microdissection, and quantitative proteomics allows the identification and characterization of molecularly distinct tissue compartments. Such workflows are typically performed using consecutive tissue sections, and so reliable sectioning and mounting of high-quality tissue sections is a prerequisite of such investigations. Embedding media facilitate the sectioning process but can introduce contaminants which may adversely affect e ...[more]

PMID: 37907539

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Dataset Information

Low-melting point agarose as embedding medium for MALDI mass spectrometry imaging and laser-capture microdissection-based proteomics

Publications

Low-melting point agarose as embedding medium for MALDI mass spectrometry imaging and laser-capture microdissection-based proteomics.

Similar Datasets

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