Rewiring of the phosphoproteome executes two meiotic divisions

ABSTRACT: The cell cycle is ordered by a controlled network of kinases and phosphatases. To generate gametes via meiosis, two distinct and sequential chromosome segregation events occur without an intervening S phase. How canonical cell cycle controls are modified for meiosis is not well understood. Here, using highly synchronous budding yeast populations, we reveal how the global proteome and phospho-proteome changes during the meiotic divisions. While protein abundance changes are limited to key cell cycle regulators, dynamic phosphorylation changes are pervasive. Our data indicate that two waves of cyclin-dependent kinase and Polo (Cdc5Polo) kinase activity drive successive meiotic divisions. These two distinct waves of phosphorylation are ensured by the meiosis-specific Spo13Meikin protein, which rewires the phospho-proteome. Spo13Meikin binds to Cdc5Polo to promote phosphorylation of a subset of substrates in meiosis I containing a newly identified motif, which we define as the Spo13Meikin -Cdc5Polo consensus phosphorylation motif. Overall, our findings reveal how a master regulator of meiosis redirects the activity of a kinase to change the phosphorylation landscape and elicit a developmental cascade.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) Saccharomyces Cerevisiae

TISSUE(S): Cell Culture

SUBMITTER: Adele Marston  

LAB HEAD: Adele Marston

PROVIDER: PXD045368 | Pride | 2026-09-12

REPOSITORIES: Pride