Targeted RNase H-mediated Extraction of crosslinked RBPs (TREX) for assessing region-specific NORAD lncRNA interactome

ABSTRACT: We used Targeted RNase H-mediated Extraction of crosslinked RBPs (TREX)to assess the endogenous binding partners of the ND4 segment of NORAD long noncoding RNA (lncRNA) in human HCT116 cells. Extracted proteins from RNase H digested and control cells (4 replicate per region per condition) were compared, using label-free (LFQ) Quantitative proteomics.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Faraz Mardakheh  

LAB HEAD: Faraz K. Mardakheh

PROVIDER: PXD045385 | Pride | 2023-12-27

REPOSITORIES: Pride