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Identification of Tufm-interacting proteins by IP-MS/MS

ABSTRACT: Tufm-FLAG and its interacting proteins were enriched by immunoprecipitation using Anti-C-Flag Nanobody Agarose Beads. Then, Tufm-FLAG-interacting proteins were identified by mass spectrum.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Brain Disease

SUBMITTER: Weiji Weng

LAB HEAD: Junfeng Feng

PROVIDER: PXD045404 | Pride | 2026-03-02

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Flag-Vector.raw	Raw
	Tufm-Flag.raw	Raw
	proteins_Flag-Vetor.csv	Csv
	proteins_Tufm_Flag.csv	Csv

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Publications

Tufm lactylation regulates neuronal apoptosis by modulating mitophagy in traumatic brain injury.

Weng Weiji W He Zhenghui Z Ma Zixuan Z Huang Jialin J Han Yuhan Y Feng Qiyuan Q Qi Wenlan W Peng Yidong Y Wang Jiangchang J Gu Jiacheng J Wang Wenye W Lin Yong Y Jiang Gan G Jiang Jiyao J Feng Junfeng J

Cell death and differentiation 20241105 3

Lactates accumulation following traumatic brain injury (TBI) is detrimental. However, whether lactylation is triggered and involved in the deterioration of TBI remains unknown. Here, we first report that Tufm lactylation pathway induces neuronal apoptosis in TBI. Lactylation is found significantly increased in brain tissues from patients with TBI and mice with controlled cortical impact (CCI), and in neuronal injury cell models. Tufm, a key factor in mitophagy, is screened and identified to be m ...[more]

PMID: 39496783

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