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LC-MS/MS data of C2C12 cells treated with Naringenin

ABSTRACT: To further clarify the reason for the upregulation of ERRγ expression by Naringenin(NAR), we biotinylated NAR for labeling. So, we used it to screen for proteins that directly interact with NAR. Lysates of C2C12 cells were co-incubated with biotin or NAR-biotin. Next, affinity cleavage magnetic beads were used to enrich the nonspecific proteins bound to biotin as well as specific proteins bound to NAR-biotin. The two sets of proteins obtained were separated by SDS-PAGE and analyzed by mass spectrometry (MS).

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Zhenyu Lv

LAB HEAD: Chang Chen

PROVIDER: PXD045449 | Pride | 2026-01-26

REPOSITORIES: Pride

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Project description:In this article, we present a data dependent acquisition (DDA) dataset which was generated as a reference and ground truth quantitative dataset. While initially used to compare samples measured with DDA and data independent acquisition (DIA) (Barkovits et al., 2020), the presented dataset holds potential value as a benchmark reference for any workflows working on DDA data. The entire dataset consists of 15 LC-MS/MS measurements composed of five distinct spike-in-states, each with three replicates. To generate the data set, a C2C12 (immortalized mouse myoblast) cell lysate was used as a complex background for five different states which were simulated by spiking 13 defined proteins at different concentrations. For this purpose, the cell lysate was used in a constant amount of 20 µg for all samples and different amounts of the 13 selected proteins ranging from 0.1 to 10 pmol were added, reflecting physiological amounts of proteins. Afterwards, all samples were tryptically digested using the same method. From each sample 200 ng tryptic peptides were measured in triplicates on a Q Exactive HF (Thermo Fisher Scientific). The mass range for MS1 was set to 350-1400 m/z with a resolution of 60,000 at 200 m/z. HCD fragmentation of the Top10 abundant precursor ions was performed at 27% NCE. The fragment analysis (MS2) was performed with a resolution of 30,000 at 200 m/z. Additionally to the raw files, the dataset contains centroided mzML files and spectrum identification results for peptide identifications performed by Mascot (Perkins et al., 1999), MS-GF+ (Kim et al., 2010) and X!Tandem (Craig and Beavis, 2004) for each separate MS analysis. The corresponding FASTA containing protein sequences as well as a combination of all identification runs performed by PIA (Uszkoreit et al., 2019, 2015) and a peptide and protein quantification performed by OpenMS (Pfeuffer et al., 2017) is included. All data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository (Perez-Riverol et al., 2018) with the dataset identifier PXD012986.

			Action	DRS
	20200103_LZY_Ctrl.raw	Raw
	20200103_LZY_LFQ_Nor.msf	Msf
	20200103_LZY_Naringenin.raw	Raw
	checksum.txt	Txt

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LC-MS/MS data of C2C12 cells treated with Naringenin

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