Proteomics

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Pseudomonas type III effector AvrRpm1 ADP-ribosylates Arabidopsis thaliana MEMBRANE-ASSOCIATED MANNITOL-INDUCED (MAMI)

ABSTRACT: Nicotiana benthamiana tissue expressing either AvrRpm1-3xFlag, StrepII-3xHA-MAMI, or co-expressing AvrRpm1-3xFlag and StrepII-3xHA-MAMI were subjected to enrichment of ADP-ribosylated proteins using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w). A non-functional Af1521 G42E mutant of the engineered Macro domain was used as control. Enriched proteins were analyzed by LC-MS/MS.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Nicotiana Benthamiana

TISSUE(S): Leaf

SUBMITTER: Lennart Wirthmueller  

LAB HEAD: Lennart Wirthmueller

PROVIDER: PXD045549 | Pride | 2023-09-28

REPOSITORIES: Pride

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