Single-cell proteomics analysis of melatonin-treated and MG132-treated in vitro post-ovulatory aged oocytes

ABSTRACT: Once ovulated, the oocyte has to be fertilized in a short time window, or it will undergo post-ovulation aging (POA), whose underlying mechanisms are still not elucidated. Here, we optimized single-cell proteomics methods and performed single-cell proteomic and phosphoproteomic analysis of fresh, POA, melatonin-treated and MG132-treated POA oocytes.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Oocyte

SUBMITTER: Yueshuai Guo  

LAB HEAD: Xuejiang Guo

PROVIDER: PXD045614 | Pride | 2025-05-06

REPOSITORIES: Pride