Dataset Information

Single-cell proteomics analysis of melatonin-treated and MG132-treated in vitro post-ovulatory aged oocytes

ABSTRACT: Once ovulated, the oocyte has to be fertilized in a short time window, or it will undergo post-ovulation aging (POA), whose underlying mechanisms are still not elucidated. Here, we optimized single-cell proteomics methods and performed single-cell proteomic and phosphoproteomic analysis of fresh, POA, melatonin-treated and MG132-treated POA oocytes.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Oocyte

SUBMITTER: Yueshuai Guo

LAB HEAD: Xuejiang Guo

PROVIDER: PXD045614 | Pride | 2025-05-06

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	GV-MII-Zygote-10ng-Boxcar-3MSX-R1-20210108.raw	Raw
	GV-MII-Zygote-10ng-Boxcar-3MSX-R2-20210108.raw	Raw
	GV-MII-Zygote-10ng-Boxcar-3MSX-R3-20210108.raw	Raw
	GV-MII-Zygote-10ng-DDA-R1-20210108.raw	Raw
	GV-MII-Zygote-10ng-DDA-R2-20210108.raw	Raw

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Publications

Single-Cell Multi-Omics Analysis of In Vitro Post-Ovulatory-Aged Oocytes Revealed Aging-Dependent Protein Degradation.

Guo Yueshuai Y Gao Mengmeng M Liu Xiaofei X Zhang Haotian H Wang Yue Y Yan Tong T Wang Bing B Han Xudong X Qi Yaling Y Zhu Hui H Situ Chenghao C Li Yan Y Guo Xuejiang X

Molecular & cellular proteomics : MCP 20241120 1

Once ovulated, the oocyte has to be fertilized in a short time window or it will undergo post-ovulation aging (POA), whose underlying mechanisms are still not elucidated. Here, we optimized single-cell proteomics methods and performed single-cell transcriptomic, proteomic, and phosphoproteomic analysis of fresh, POA, and melatonin-treated POA oocytes. POA oocytes showed downregulation of most differentially expressed proteins, with little correlation with mRNA expression, and the protein changes ...[more]

PMID: 39571909

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