Identification of G-quadruplex-interacting proteins in living cells using an artificial G4-targeting biotin ligase

ABSTRACT: G-quadruplexes (G4s) are noncanonical nucleic acid structures pivotal to cellular processes and disease pathways. Deciphering G4-interacting proteins is imperative for unraveling G4's biological significance. In this study, we developed a G4-targeting biotin ligase named G4PID, meticulously assessing its binding affinity and specificity both in vitro and in vivo. Capitalizing on G4PID, we devised a tailored approach termed G-quadruplex-interacting proteins specific biotin-ligation procedure (PLGPB) to precisely profile G4-interacting proteins.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Ziang Lu  

LAB HEAD: Xiang Zhou

PROVIDER: PXD045634 | Pride | 2024-05-24

REPOSITORIES: Pride