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Plasma membrane deformation by one-dimensional wetting triggers the formation of a branched F-actin network in endothelial cells

ABSTRACT: Membrane deformation by one-dimensional (1D) wetting, a physical process driven by the interaction of the plasma membrane with adhesive nanofibers, triggers the formation of filopodium-like plasma membrane protrusions. In these protrusions, F-actin reorganization occurs after membrane deformation. Here, we interrogate the molecular mechanisms underlying F-actin reorganization in such protrusions formed by human primary epithelial and endothelial cells. While generally assumed that filopodium-like protrusions encompass F-actin bundles, as we observed in epithelial cells, cryo-electron tomography unexpectedly revealed a branched F-actin network in 1D wetting protrusions formed by endothelial cells. Combining high resolution photonic microscopy approaches with genetic and drug perturbations, we show that Arp2/3 activity is required for the proper reorganization of F-actin in 1D wetting endothelial protrusions, a process regulated through the local activation of Cdc42 and N-WASP. Our data suggest that F-actin reorganization in response to membrane deformation by 1D wetting is differentially regulated in epithelial versus endothelial cells, possibly impacting a wide range of physiological processes, such as cell migration, adhesion, and microenvironment probing.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Neisseria Meningitidis Serogroup C (strain 8013)

SUBMITTER: Magalie Duchateau

LAB HEAD: Guilllaume Dumenil

PROVIDER: PXD045648 | Pride | 2026-05-13

REPOSITORIES: Pride

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			Action	DRS
	20221130_Q2_MD_ColQ2-117_Margot_I_Arp_1.raw	Raw
	20221130_Q2_MD_ColQ2-117_Margot_I_Arp_2.raw	Raw
	20221130_Q2_MD_ColQ2-117_Margot_I_Arp_3.raw	Raw
	20221130_Q2_MD_ColQ2-117_Margot_I_CDC_1.raw	Raw
	20221130_Q2_MD_ColQ2-117_Margot_I_CDC_2.raw	Raw

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Plasma membrane deformation by one-dimensional wetting triggers the formation of a branched F-actin network in endothelial cells

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