ANP32B is a macroh2A histone chaperone
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER: 
Imke Mandemaker
LAB HEAD: Andreas Ladurner
PROVIDER: PXD045696 | Pride | 2023-10-24
REPOSITORIES: Pride
Publications
The histone chaperone ANP32B regulates chromatin incorporation of the atypical human histone variant macroH2A.
Mandemaker Imke K IK Fessler Evelyn E Corujo David D Kotthoff Christiane C Wegerer Andreas A Rouillon Clément C Buschbeck Marcus M Jae Lucas T LT Mattiroli Francesca F Ladurner Andreas G AG
Cell reports 20231019 10
All vertebrate genomes encode for three large histone H2A variants that have an additional metabolite-binding globular macrodomain module, macroH2A. MacroH2A variants impact heterochromatin organization and transcription regulation and establish a barrier for cellular reprogramming. However, the mechanisms of how macroH2A is incorporated into chromatin and the identity of any chaperones required for histone deposition remain elusive. Here, we develop a split-GFP-based assay for chromatin incorpo ...[more]
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