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Single blood donor causing multiple allergic and anaphylactic transfusion reactions

ABSTRACT: Allergic transfusion reactions (ATRs) occur within 24 h of transfusion. A less common but more severe manifestation of ATR is anaphylaxis. The relationship between the mechanisms underlying ATRs and donor, product, and/or recipient factors is not well understood. Our aim is to investigate the underlying factors responsible for eliciting anaphylactic ATRs and possibly identify the molecule responsible, that could routinely be used as a biomarker in subsequent pre-transfusion screens. We employ an IP-MS workflow for this purpose. Purified IgG antibodies from donor plasma are immobilized on magnetic beads and interacted with basophil cells lysates. The immunoprecipitants are analyzed by mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lad-2 Cell

SUBMITTER: Michael Wierer

LAB HEAD: Morten Hanefeld Dziegiel

PROVIDER: PXD045721 | Pride | 2026-06-08

REPOSITORIES: Pride

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Publications

Severe allergic and anaphylactic transfusion reactions in consecutive recipients from the same donor.

Brooks Patrick Terrence PT Mikkelsen Christina C Bartko Ewa Anna EA Ravikumar Vaishnavi V Hother Christoffer Egeberg CE Minculescu Lia L Vibeke Hanne H Ostrowski Sisse Rye SR Hansen Morten Bagge MB Poulsen Lars K LK Blom Lars H LH Garvey Lene H LH Dziegiel Morten Hanefeld MH

Haematologica 20251127 5

PMID: 41307136

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