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S. aureus peptidoglycan hydrolysis by mutanolsyin

ABSTRACT: (Manuscript abstract) "Peptidoglycan, a gigadalton polymer, functions as the scaffold for bacterial cell walls and provides cell integrity. Peptidoglycan is remodelled by a large and diverse group of peptidoglycan hydrolases, which control bacterial cell growth and division. Over the years, many studies have focused on these enzymes, but knowledge on their action within peptidoglycan mesh from a molecular basis is scarce. Here, we provide structural insights into the interaction between short peptidoglycan fragments and the entire sacculus with two evolutionarily related peptidases of the M23 family, lysostaphin and LytM. Through nuclear magnetic resonance, mass spectrometry, information-driven modelling, site-directed mutagenesis and biochemical approaches, we propose a model in which peptidoglycan cross-linking affects the activity, selectivity and specificity of these two structurally related enzymes differently."

INSTRUMENT(S): Synapt MS

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Alicja Razew

LAB HEAD: Izabela Sabala

PROVIDER: PXD045856 | Pride | 2023-11-06

REPOSITORIES: Pride

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S. aureus peptidoglycan hydrolysis by mutanolysin and LytM catalytic domain

Project description:(Manuscript abstract) "Peptidoglycan, a gigadalton polymer, functions as the scaffold for bacterial cell walls and provides cell integrity. Peptidoglycan is remodelled by a large and diverse group of peptidoglycan hydrolases, which control bacterial cell growth and division. Over the years, many studies have focused on these enzymes, but knowledge on their action within peptidoglycan mesh from a molecular basis is scarce. Here, we provide structural insights into the interaction between short peptidoglycan fragments and the entire sacculus with two evolutionarily related peptidases of the M23 family, lysostaphin and LytM. Through nuclear magnetic resonance, mass spectrometry, information-driven modelling, site-directed mutagenesis and biochemical approaches, we propose a model in which peptidoglycan cross-linking affects the activity, selectivity and specificity of these two structurally related enzymes differently."

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Dissociation of β2m from MHC Class I triggers formation of noncovalent, transient heavy chain dimers

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Mycobacterium tuberculosis protein production pipeline

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Lipases from Metagenomic Sources

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The marine diterpenes pseudopteroxazole (Ptx) and pseudopterosin G (PsG) target the integrity of the gram-positive cell wall

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