Etomoxir as a Promiscuous Chemoproteomic Probe.

ABSTRACT: Primary hepatocytes from WT or Pex5KO mouse livers were treated with 100uM clickable etomoxir analogues and the proteins bound to etomoxir were pulled down and identified. The small molecule inhibitor etomoxir has been used for many years as a specific inhibitor of Cpt1 but these data show that etomoxir binds a large array of proteins and is not appropriate as a tool for evaluating the biological effects of fatty acid oxidation. Primary hepatocytes were treated with 100uM clickable etomoxir analogues and the proteins bound to etomoxir were pulled down and identified. The small molecule inhibitor etomoxir has been used for many years as a specific inhibitor of Cpt1 but these data show that etomoxir binds a large array of proteins and is not appropriate as a tool for evaluating the biological effects of fatty acid oxidation.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Primary Cell, Hepatocyte

SUBMITTER: Michael Wolfgang  

LAB HEAD: Michael J. Wolfgang

PROVIDER: PXD045866 | Pride | 2024-10-17

REPOSITORIES: Pride