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Proteomic analysis of vitreous in WT and CD11b KO mice.

ABSTRACT: Our data support that following optic nerve crush injury, ocular infiltration of immune cells causes damage to the retinal vasculature and compromises the integrity of the blood-retina barrier (BRB). Functional ablation of integrin alphaM (CD11b) attenuates immune cell trafficking, protects the BRB, and strongly enhances β-glucan-elicited retinal ganglion cells axon regeneration. Here, we performed an unbiased analysis of the vitreal proteome; we harvested vitreous humor from WT and Itgam-/- mice, β-glucan treated after optic nerve injury, and used mass spectrometry-based proteomics for differential analysis of the WT and Itgam-/- vitreous proteome (label free and tandem mass tagging).

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Vitreous Humor

SUBMITTER: Juan Oses-Prieto

LAB HEAD: Roman J Giger

PROVIDER: PXD045885 | Pride | 2026-08-05

REPOSITORIES: Pride

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			Action	DRS
	S20210915-05.raw	Raw
	S20210915-07.raw	Raw
	S20211022-03.raw	Raw
	S20211022-05.raw	Raw
	S20211118-16_FTMSms2hcd.raw	Raw

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Publications

Neutrophil-inflicted vasculature damage suppresses immune-mediated optic nerve regeneration.

Passino Ryan R Finneran Matthew C MC Hafner Hannah H Feng Qian Q Huffman Lucas D LD Zhao Xiao-Feng XF Johnson Craig N CN Kawaguchi Riki R Oses-Prieto Juan A JA Burlingame Alma L AL Geschwind Daniel H DH Benowitz Larry I LI Giger Roman J RJ

Cell reports 20240315 3

In adult mammals, injured retinal ganglion cells (RGCs) fail to spontaneously regrow severed axons, resulting in permanent visual deficits. Robust axon growth, however, is observed after intra-ocular injection of particulate β-glucan isolated from yeast. Blood-borne myeloid cells rapidly respond to β-glucan, releasing numerous pro-regenerative factors. Unfortunately, the pro-regenerative effects are undermined by retinal damage inflicted by an overactive immune system. Here, we demonstrate that ...[more]

PMID: 38492223

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