Dataset Information

Proteomic analysis of human sperm protamines from men with and without infertility

ABSTRACT: The objective of this study was to provide a comprehensive assessment of protamine isoforms and modifications in human sperm with the aim of identifying how protamine modifications/isoforms are altered in men with reduced sperm motility and low sperm count. Sperm samples from 18 men with prior reported pregnancy and normozoospermia 14 men from couples with infertility and asthenozoospermia and 24 men from couples with infertility and oligoasthenoteratozoospermia were included in the study. Proteomic assessment using both top-down and bottom-up LC-MS was performed.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Sperm

SUBMITTER: Samantha Schon

LAB HEAD: Samantha Schon

PROVIDER: PXD046041 | Pride | 2025-05-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MSB53637Chym112621.raw	Raw
	MSB53637Chym112621.raw_20211208_Byonic.mzid.mzid.gz	Mzid
	MSB53637Chym112621.raw_20211208_Byonic.mzid.mzid_MSB53637Chym112621.raw_20211208_Byonic.mzid.MGF	Mzid
	MSB53637Elas112621.raw	Raw
	MSB53637Elas112621.raw_20211209_Byonic.mzid.mzid.gz	Mzid

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Publications

Proteomic analysis of human sperm reveals changes in protamine 1 phosphorylation in men with infertility.

Schon Samantha B SB Moritz Lindsay L Rabbani Mashiat M Meguid Julia J Juliano Brock R BR Ruotolo Brandon T BT Aston Kenneth K Hammoud Saher Sue SS

F&S science 20231207 2

<h4>Objective</h4>To perform a comprehensive assessment of protamine (P) isoforms and modifications in human sperm with the aim of identifying how P modifications and isoforms are altered in men with reduced sperm motility and low sperm count.<h4>Design</h4>Cross-sectional.<h4>Setting</h4>Academic medical center.<h4>Patients</h4>A total of 18 men with prior reported pregnancy and normozoospermia (normal sperm), 14 men from couples with infertility and asthenozoospermia (reduced sperm motility), ...[more]

PMID: 38065301

Similar Datasets

Project description:Infertility is a widespread problem, affecting around 15% of couples worldwide, and is defined as the inability to achieve pregnancy within one year despite unprotected intercourse 1. Infertility can be caused by either male or female reproductive issues. Various medical conditions including malignancies, infections, urogenital conditions, or genetic causes can contribute to male infertility. However, 30-40% of men in their reproductive age are affected by idiopathic infertility, according to the guidelines of European Association of Urology (EAU) 1. Towards a better understanding of male infertility, it is mandatory to achieve a comprehensive understanding of involved genes, their RNA transcripts, and regulatory factors, including miRNAs, which influence the expression level of proteins. Therefore, such proteins need to be identified to investigate their role in spermatogenesis and male infertility. Although there are numerous studies on RNAs, including miRNAs related to male infertility 2-9, there are few studies aiming to cover the whole proteome of human sperm 10. The sperm transcriptome comprises a total of 60,505 transcripts including 11,688 differentially expressed transcripts in infertile and fertile men, as reported by Joshi et al. (2022)11. The entire sperm proteome encompasses 6871 proteins, as summarized by Castillo et al. (2018)10. Nevertheless, there is still a lack of high-throughput studies aiming to identify dysregulated proteins in sperm from subfertile men. Only few studies focused on comparisons of the sperm proteome in men with asthenozoospermia and there is virtually no proteomic studies of oligoasthenozoospermic men 12. Some identified proteins in sperm have functions in maintaining sperm motility and enabling fertilization and are involved in structural composition and/or energy metabolism 12-14 and others are not yet functionally characterized. In this study, we employed Mass spectrometry (MS) technology that is still rarely used in the field of human reproductive research to investigate the proteomic landscape of human sperm and their differential expression patterns in men with subfertility.